RE: [Histonet] How to stain mast cell in mouse lung sections with toluidine blue?

From:"Marshall Terry Dr, Consultant Histopathologist"

CD 117 rules, (but I far prefer looking at aldehyde fuchsin).

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: Kemlo Rogerson []
Sent: 12 July 2006 15:40
To: 'min he';
Subject: RE: [Histonet] How to stain mast cell in mouse lung sections
withtoluidine blue?

Mast cells, as you know, contain heparin, histamine and 5 hydroxytryptamine
and many people suggest that alcohol fixation may be more prudent for the
preservation of the granules. Basic lead acetate fixation is recommended but
some do NOT recommend formol fixation as it is thought you need a heavy

The presence of the sulphated polysaccharide heparin is responsible for
staining with the thiazin dyes down to pH 0.5 to 1. So I assume variations
in the amounts of the sulphated polysaccharide heparin may account for your

You may like to try other techniques such as Alcian Blue PAS, Acid
diazosafranin or other stains such as Biebrich scarlet, aldehyde fuchsin,
etc. I assume it's like most things in life there is no common mast cell.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
Pager 07659 597107

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