RE: [Histonet] Giemsa Stain

From:Kemlo Rogerson

With respect you have no books over there?

Giemsa; methylene blue eosinate 4g, azure B eosinate 5g, azure A eosinate 1g
and methylene blue chloride (85 to 88% dye content) 2g; keep as dry stain in
a cool dry place tightly stoppered. The solvent is an equal volume of 100%
ethyl alcohol and 98% glycerol and the dye is dissolved 800mg/ 100ml of the
mixed solvent.

Light green? What for? Light green has a molecular weight of 792.863 CI of
42095 and dissolves in water at 20g per 100 mls and in alcohol at 0.82 g per
100 mls. In Masson's stain a 2% aqueous light green in 1% acetic acid
solution is used. 

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
Pager 07659 597107

The real voyage of discovery consists not in seeing new landscapes, but in
having new eyes. --Marcel Proust 

This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your


Histonet mailing list

<< Previous Message | Next Message >>