[Histonet] tissue loss

From:Melissa Mazan

Hi - I'm trying a citrate buffer antigen retrieval protocol.  After I 
have dewaxed the slides, I put them in citrate buffer - protocol says 
microwave on high for 25 minutes.  However, when I remove the slides all 
the tissue has slid off the slide.  Any suggestions? Melissa
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor of Large Animal Medicine
Director of Sports Medicine
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA 01536
Tel: 508-839-5395
Fax: 508-839-7922
Email: melissa.mazan@tufts.edu

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