[Histonet] help with Technovit 8100 embedding/section


Dear members, I am trying to view in vivo transfected GFP expression in mouse testis so used glycol 
methacylate(Technovit 8100) to embed the testis after fixation, acetone dehydration and
infilitration.  The polymerized tissue-block was too hard and crisp to cut, the microtome
experienced extreme shattering. I was only able to get shatters instead of tissue section.   Any
comments and suggestion is greatly appreciated!

Huanghui Tang
Northwestern University

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