[Histonet] antibodies/protocols for oligodendrocytes & microglia
If there is anyone out there doing IHC/IF on primate free-floating
40um cryostat sections
fixed in Zamboni fixative for Oligodendrocytes & Microglia - our lab
would very much like
to hear from you!!! Even though we have been successful doing IHC/IF
for other markers,
we have had NO success with the two above markers.
We are in need of working protocol(s) & successful antibodies for
Microglia. We have tried several antibodies for IHC/IF from several
companies such as:
Chemicon's anti-CNpase for oligodendrocytes - no staining.
LabVision's mylein base protein (a protein that is expressed in
it stained the mylein sheath but not the cell bodies.
Biocare Medical's microglia (Iba1) antibody - also without success.
I can tell you its been very frustrating trying various antibodies/
protocols - all without
success on our primate sections. Please, we would greatly appreciate
helpful tips & equally important share your working protocol(s). We
also want to hear
from companies too.
Maria Bartola Mejia
Department of Neurosurgery
University California San Francisco
San Francisco, Ca 94103
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