[Histonet] affixing large tissue blocks to glass at room temperature
I have several mouse liver "slices" that are fixed in NBF.
Basically, I took a fresh liver, immersed it in NBF for about an
hour, then sliced a lobe like a loaf of bread. I then put it in fix
overnight to make sure the inside of the slices were well fixed. I
now want to take "whole mount" pictures of the slices. It would help
immensely if I could somehow fix the slices to a glass plate. The
problem is that I want to make sure I don't destroy the slices as I
might want to section them later. Are there any suggestions for
temporarily affixing the slices to a glass plate at room
temperature? Someone suggested double sided tape, but of course this
could damage the tissue.
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