[Histonet] affixing large tissue blocks to glass at room temperature

From:"Caroline Bass"

Hey guys,

I have several mouse liver "slices" that are fixed in NBF.   
Basically, I took a fresh liver, immersed it in NBF for about an  
hour, then sliced a lobe like a loaf of bread.  I then put it in fix  
overnight to make sure the inside of the slices were well fixed.  I  
now want to take "whole mount" pictures of the slices.  It would help  
immensely if I could somehow fix the slices to a glass plate.  The  
problem is that I want to make sure I don't destroy the slices as I  
might want to section them later.  Are there any suggestions for  
temporarily affixing the slices to a glass plate at room  
temperature?  Someone suggested double sided tape, but of course this  
could damage the tissue.



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