[Histonet] Troubleshoot on mounting tissue
This is my first time using this listserve, so I hope this works.
I have a question about mounting tissues (PFA perfused rat brain tissues to
be specific). I processed them for IHC using ABC complex system and DAB and
used deionized water as my mounting medium. However, when I put the tissue
in water, it swells tremendously and easily becomes wrinkled. I think as a
consequence of the swelling, there are holes throughout my tissues. (I
didn't use hydrogen peroxide until DAB, and it was at an extremely low
concentration of less than 0.1%.)
Anyways, is my hypothesis plausible (that mounting my tissue in DI water can
cause tissues to swell and create holes)? If so, does anyone know how I can
eliminate this problem? Or can it be another factor (bad cryoprotectant,
bad perfusion, bad buffer)? I appreciate any feedback I can get.
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