Folks,

We are having a heck of a time keeping mouse rib on a slide during
antigen retrieval and IHC. Currently we are cutting the rib on gold plus
slides and baking at 60 C for 1 hour. Then the slides are air dried for
several days before staining. This works fine for our human can canine
bone and for mouse femur. However, the amount of bone on these mouse
ribs is so thin, comes right off.

Any suggestions?

Thanks!!

Julie

Julie Randolph-Habecker, Ph.D.
Staff Scientist - Manager
Experimental Histopathology Shared Resource
Fred Hutchinson Cancer Research Center
1100 Fairview Ave, N. M5-A803
Seattle WA 98109-1024
206-667-6119
jhabecke@fhcrc.org

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