[Histonet] Re: Giemsa Stain
Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using the
powder. I also need a recipe for making light green from powder.<<
I've never - in more than 40 years - encountered anyone - including an
intensely giemsophilic research lab 40 years ago - Wolbach's colophonium rosin and
all - who made up their own Giemsa stain solution from the dry powder. It's
supposed to be extremely difficult to do. Scrupulous exclusion of water is
essential - that applies to stock bottles of the ready-made solution also.
Kemlo has given you adequate advice about Light Green SF. - And yes, Kemlo,
many labs on this side of the pond have no books. American science education
marches on into the new century.
Bob Richmond
Knoxville TN and Gastonia NC
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