Hi Atoska:

    Hydrocephalus is not uncommon in mice, perhaps that is the source of 
your concern? I suppose it could be manifested more on one side than the 
other? Maybe you could post a photo?

Geoff

Atoska S. Gentry wrote:

>  Hello, please have any of you who process & section paraformaldehyde 
> fixed, paraffin embedded mouse brain experienced asymmetry of the 
> right & left hemispheres? Recently our coronal mouse brain sections 
> which appear symmetrical upon gross trim and after initial facing of 
> paraffin are asymmetrical after staining. I've rotated the block 
> holder to the limit and sectioned the most rostral hemisphere at 
> higher micron thickness to accommodate. However, to my dismay and 
> disappointment none of this has helped very much. Any pointers in 
> remedying this situation ASAP will be greatly appreciated. Thanks, Atoska
>
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-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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