You can just add your strep-HRP or ABC step to a section (without primary
or secondary) and if you get signal it is surely from the biotin
endogenous to the tissue. It is a good control to run in any given
experiment.

Biotin is tricky as it often appears in very "specific" patterns and is
often misinterpreted as real staining.

I also agree HIER can dramatically increase biotin signal.

> ----- Original Message -----
> From: "Gudrun Lang" 
> To: "Histonetliste (Histonetliste)" 
> Sent: Wednesday, July 27, 2005 3:58 PM
> Subject: [Histonet] endogen biotin in prostata?
>
>
>> Hi,
>>
>> Since a few weeks our 34betaE12 shows granular spots in the cytoplasma
>> of
>> some cells in the prostata-slide. They look really sprinkled, but not in
>> all. Could that be endogen biotin?
>>
>> Or appears biotin more homogen? Please help an unknowing histotech.
>>
>>
>>
>> Gudrun Lang
>>

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