Re: [Histonet] endogen biotin in prostata?
You can just add your strep-HRP or ABC step to a section (without primary
or secondary) and if you get signal it is surely from the biotin
endogenous to the tissue. It is a good control to run in any given
Biotin is tricky as it often appears in very "specific" patterns and is
often misinterpreted as real staining.
I also agree HIER can dramatically increase biotin signal.
> ----- Original Message -----
> From: "Gudrun Lang"
> To: "Histonetliste (Histonetliste)"
> Sent: Wednesday, July 27, 2005 3:58 PM
> Subject: [Histonet] endogen biotin in prostata?
>> Since a few weeks our 34betaE12 shows granular spots in the cytoplasma
>> some cells in the prostata-slide. They look really sprinkled, but not in
>> all. Could that be endogen biotin?
>> Or appears biotin more homogen? Please help an unknowing histotech.
>> Gudrun Lang
Histonet mailing list
<< Previous Message | Next Message >>