Re: [Histonet] Problems of processing brain for paraffin embedding

From:John Kiernan

Dear A.P.Tafreshi,

Have you discussed your problems with an experienced
colleague? Someone in the histopathology department
of a nearby hospital could probably advise you.

Your statement, "I postfixed them in the same buffer"
indicates that you mmy not know what the words "fix" and
"buffer" mean. You need on-the-spot advice, and it will
be available in abundance in your own city.

Histotechnicians worldwide are generous in giving 
advice to colleagues. Visit a local lab, and meet the
people who will give you advice that's based on a
combination of education, training and practical 

John Kiernan
London, Canada.
azita parvaneh tafreshi wrote:
> Dear Members,
> I have problems with paraffin processing of mouse and hamster brains and spinal cords. I have perfused the animals with PFA 4% and I postfixed them in the same buffer for 7-8 days and then stored in Alc 70% (up to now). After sectioning, tissues wrinkle in a way that it is torn after flattenning (on a slide warmer), therefore the quality is not good at all.
> Can anyone give me hints to overcome the problem. A detailted protocol of paraffin processing would be great.
> Regard
> A.P. Tafreshi

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