Which antibody are you using?  We have had great success with Santa Cruz (goat
polyclonal) until I ordered a new lot this past month.  When I called them, they
indicated to me that they were having problems with their goat, literally.  I
guess it had stopped producing antibodies to CD31 that work in IHC
(interestingly enough, they still work in Westerns).  They hope to have the
problem resolved within the next month, with a new goat.

Angela McNabola, MS, HT(ASCP)SLS, QIHC
Bayer Healthcare
400 Morgan Lane
West Haven, CT 06516


                                                                                                                                              
                      "Kim Allred"                                                                                                            
                                     To:                                            
                      Sent by:                              cc:                                                                               
                      histonet-bounces@lists.utsouth        Subject:  [Histonet] CD 31                                                        
                      western.edu                                                                                                             
                                                                                                                                              
                                                                                                                                              
                      07/22/2005 03:15 PM                                                                                                     
                                                                                                                                              
                                                                                                                                              




I am having a problem getting my CD31 to work.  The protocol was for an
overnight incubation and pepsin pretreatment.  I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab.  Does anyone have any ideas?  This seemed to
work well for a while but once again, I'm having problems with it.  Any
input would be appreciated.  Thanks in advance!







Kim Allred

Dermatopathology Associates

Jackson, MS

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