Re: [Histonet] CD 31

From:Lori Richey

We use DAKO CD31 1:25 , 18 minutes microwave, in citrate buffer.

Kim Allred wrote:

>I am having a problem getting my CD31 to work.  The protocol was for an
>overnight incubation and pepsin pretreatment.  I couldn't get it to work
>this way so I altered it a bit by using trilogy for AR and a two hour
>incubation for the primary ab.  Does anyone have any ideas?  This seemed to
>work well for a while but once again, I'm having problems with it.  Any
>input would be appreciated.  Thanks in advance!  
>
> 
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>Kim Allred 
>
>Dermatopathology Associates
>
>Jackson, MS  
>
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>Histonet@lists.utsouthwestern.edu
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>  
>

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