We use DAKO CD31 1:25 , 18 minutes microwave, in citrate buffer.

Kim Allred wrote:

>I am having a problem getting my CD31 to work.  The protocol was for an
>overnight incubation and pepsin pretreatment.  I couldn't get it to work
>this way so I altered it a bit by using trilogy for AR and a two hour
>incubation for the primary ab.  Does anyone have any ideas?  This seemed to
>work well for a while but once again, I'm having problems with it.  Any
>input would be appreciated.  Thanks in advance!  
>
> 
>
> 
>
> 
>
>Kim Allred 
>
>Dermatopathology Associates
>
>Jackson, MS  
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet