RE: [Histonet] "waviness"

From:"Lorraine Cornett"

Julie, we have had this problem in the past as well. One thing to look at, 
especially with using charged slides, is to make absolutely sure that the 
slides are DRY, no water at all, before staining.  Are you using a auto 
stainer or hand staining?  Somtimes we noticed that water was being trapped 
under the paraffin (had a bad lot of charged or plus slides) and would 
usually result in the pathologist complaining about waviness.  Make sure you 
water bath temps are warm enough to flatten the sections before picking up 
as well.  Keep me posted as to what resolves it for you.  You never know, 
this monster may show back up in my lab as well.



Lorraine Cornett, HT (ASCP)
Highlands Pathology
Blue Ridge Division, Kingsport, TN
423 224-5793 fax 423 224-5349




>From: Julie.Sanders@med.va.gov
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] "waviness" Date: Thu, 28 Jul 2005 06:46:21 -0500
>
>
>One of our pathologists is complaining about having to focus up and down 
>too
>much while reading slides.  I have tried many things to resolve the problem
>but nothing is making him happy.  I have changed the way we pick up from
>water bath to be sure slides are perpendicular (straight up and down) when
>picking up sections, we use only plus slides, I've asked that slides not be
>placed on the warming platform after picking up.  Does anyone have any 
>ideas
>about how I can fix this problem?
>
>Julie
>
>Julie Sanders
>Supervisor, Anatomic Pathology
>Cincinnati VA
>
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