At any given temperature, tissues infiltrated with sucrose tend to be softer
and less completely frozen than similar tissues without sucrose (and 30% is
a very high level of sucrose), and therefore they compress and wrinkle more.
Try cutting them at a colder temperature than you would normally use for
liver.  That will probably help produce flatter sections with less
wrinkling.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of
> Steven Coakley
> Sent: 	Friday, July 22, 2005 12:27 PM
> To: 	Histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] cryosectioning liver/sucrose
> 
> Something a "tad" new for me here.  I've never sectioned 4%Para liver
> infiltrated with 30% sucrose.  They sectioned well but the slide appear to
> be loaded with what appears to be folds throughout.  Not what I routinely
> see on regular unfixed FS.  Is this normal for fixed sucrose infiltrated
> sections.  Are there any special techniques when dealing with this special
> tissue.
>  
> Steve
> 
> 
> 		
> ---------------------------------
>  Start your day with Yahoo! - make it your home page 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet