RE: [Histonet] Problem with Eosin Y staining

From:"Joyce Cline"

We have a high humidity problem and I use H-2 Blue beads to remove water
from our clearing agent. We check and change our alcohols after our
eosin everyday. Our clearing agent will be opaque if not checked
frequently. (water in the clearing agent will cause Eosin to bleed)

Our laboratory is having a problem with the staining of mouse tumor
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going
fine until recently.  The specific problem we are having is after the
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to
be bleeding out, leaving only the hemotoxylin staining.  A histologist
from the institute suggested it may have something to do with the
relative humidity in our lab, and therefore allowing the slides to set
under a flow-hood may help.  Any other suggestions or protocol
modifications would be greatly appreciated.  Thank you very much.

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