RE: [Histonet] CD 31
Hi Kim,
We use CD31 at 1:50, pretreatment HIER using Citrate buffer
Rena Fail
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim
Allred
Sent: Friday, July 22, 2005 3:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD 31
I am having a problem getting my CD31 to work. The protocol was for an
overnight incubation and pepsin pretreatment. I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab. Does anyone have any ideas? This seemed
to
work well for a while but once again, I'm having problems with it. Any
input would be appreciated. Thanks in advance!
Kim Allred
Dermatopathology Associates
Jackson, MS
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