[Histonet] mixing up primary and secondary antibodies for double/triple staining
I have a general question on double or triple fluorescence stainings.
Jackson Immunoresearch recommends doing sequential stainings, i.e.
complete staining for antigen A first, then antigen B and then antigen
C. Is there any danger in mixing up all the primary antibodies in one
mix instead of using the sequential approach ? And, if using secondary
antibodies derived from the same species, is there any danger in doing
the same for the secondaries ?
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