[Histonet] culture on coverslips troubles
Greetings Histonetters:
I'm being asked to give advice about how to keep cultured cells on their cover slips during IHC for Confocal Microscopy. Is there a specific technique for washing the cover slips without losing cells? Should the cover slips be "air-dried" at some point? What fixative is best for confocal microscopy? Are there positively charged cover slips out there? Last question: Does anyone know of a list server for culture people? Marketing people please feel free to respond to me regarding these questions if you think you can be of service. I thank you all, and I send thanks for my questioning friend.
LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave. CSC K4/913
Madison, Wisconsin 53792
Phone: 608-262-3518
FAX: 608-263-3746
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