[Histonet] background on the negative controls
Has anyone had a problem on their negative controls having heavy
brown staining that looks like real DAB staining on negative mouse and
Rabbit controls that were retirieved using heat but the negative
control that used only protease treatment was very clean. I did a lot
of IHCs on adrenal mass tissue and my Rabbit and Mouse negatives look
like they have positive staining. H2O2 treatment did not correct the
problem, I also used the Avidin Biotin block. Anymore suggestions?
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