[Histonet] anti-talin antibody

From:"Elizabeth Chlipala"

Hello everyone
 
I have been working on an Immunohistochemical protocol for talin on
human skin samples.  I currently have the chemicon antibody.  I have
tried it on both frozen and paraffin sections, but I'm not having much
success.  I get minimal staining with retrieval in the paraffin
sections.  When I tried the frozen sections I got no staining at all.
Has anyone used this antibody before?  Any help would be appreciated,
different antibody source, etc.
 
Thanks
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai

<< Previous Message | Next Message >>