[Histonet] Under-Decal'd tissue
Trisha - I swear by RDO, manufactured by Apex Engineering Products Corporation, Aurora, IL. The active ingredient is hydrochloric acid. If a block still contains calcification after either preliminary decalcification or if discovered after processing/facing, I drop the block into a small beaker with RDO and leave it for from 15-30 minutes (depending upon bone density). I've routinely used RDO for years on normal bony blocks, trephines, bone bx's, etc. and it works amazingly well. It is NOT, however, for LONG-TERM decalcification (i.e., overnight) because it works so well that it may render the bone devoid of any detail if left too long. The RDO must be washed off the block with a good dose of water before cutting, as the acid will discolor your knife holder.
Their number is 800-451-6291 or you can contact them at www.rdo-apex.com.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Saturday, July 23, 2005 11:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 31
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When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. 2"x3" slides (Angela Bitting)
2. Specimen Database (Paula Pierce)
3. RE: specimen database (Bonner, Janet)
4. Re: 2"x3" slides (Gayle Callis)
5. Re: Endogenous peroxidase blocking (Pablo S?nchez Quinteiro)
6. Feedback on Fc Blocker and Background buster for Ventana IHC
Machines (Nava, Josefa)
7. Re: specimen database (K.Bowden)
8. AW: [Histonet] 2"x3" slides (Gudrun Lang)
9. background on the negative controls (Nava, Josefa)
10. Free Histology Learning Resources (LINDA MARGRAF)
11. RE: Limit on amount of formalin you can pour down sink?
(mprice26@juno.com)
12. Re: RE: Limit on amount of formalin you can pour down sink?
(Jackie M O'Connor)
13. CD 31 (Kim Allred)
14. RE: CD 31 (renafail@bellsouth.net)
15. cryosectioning liver/sucrose (Steven Coakley)
16. Re: Black paraffin and cassettes (Mark Tarango)
17. RE: RE: Limit on amount of formalin you can pour down sink?
(Bonner, Janet)
18. RE: cryosectioning liver/sucrose (Monfils, Paul)
19. under decal (Trisha Emry)
20. RE: under decal (Bonner, Janet)
21. RE: under decal (Monfils, Paul)
22. Re: CD 31 (Lori Richey)
23. Staining for Helicobacter pylori (IamNinaOwen@aol.com)
24. Re: Staining for Helicobacter pylori (Drew Meyer)
----------------------------------------------------------------------
Message: 1
Date: Fri, 22 Jul 2005 13:00:16 -0400
From: "Angela Bitting"
Subject: [Histonet] 2"x3" slides
To:
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Does anyone out there have a good way to stain 2"x3" glass slides (whole-mount prostates) using a Sakura stainer? I have a 2000 and 601 model and have tried adapting the rack so I can stain 4 slides at a time, but it's so inefficient. Any ideas?
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
------------------------------
Message: 2
Date: Fri, 22 Jul 2005 10:07:53 -0700 (PDT)
From: Paula Pierce
Subject: [Histonet] Specimen Database
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050722170753.97109.qmail@web50305.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I created my entire patient database myself in Microsoft Access.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
405-759-3953
contact@excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 3
Date: Fri, 22 Jul 2005 13:18:13 -0400
From: "Bonner, Janet"
Subject: RE: [Histonet] specimen database
To: "'Janella Seaton '" ,
"'histonet-bounces@lists.utsouthwestern.edu '"
,
"'histonet@lists.utsouthwestern.edu '"
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4332@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
We're using Copath with alot of success - it is very user-friendly.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: 7/22/2005 11:58 AM
Subject: [Histonet] specimen database
Hello!
We want to implement a database for specimens we receive in our lab.
We would use this database starting with logging in specimens and ending
with archiving of specimens. It would be used to track when various
steps
(gross photos, processing, staining, etc) have occurred and by whom.
We do want a system that is user friendly.
Any suggestions or advice?
Thanks for the help.....
j.s.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Fri, 22 Jul 2005 11:53:51 -0600
From: Gayle Callis
Subject: Re: [Histonet] 2"x3" slides
To: "Angela Bitting" ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050722115129.01b58ce8@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Years ago, I think the Shandon stainer had a rack to accomodate the larger
slides, maybe Sakura needs to do this too. Otherwise, and we still do it
now - hand staining, and can do either 20 or 30 at a time - kinda old
fashioned but very efficient.
At 11:00 AM 7/22/2005, you wrote:
>Does anyone out there have a good way to stain 2"x3" glass slides
>(whole-mount prostates) using a Sakura stainer? I have a 2000 and 601
>model and have tried adapting the rack so I can stain 4 slides at a
>time, but it's so inefficient. Any ideas?
>
>Angela Bitting, HT(ASCP)
>Technical Specialist, Histology
>Geisinger Medical Center
>100 N Academy Ave. MC 23-00
>Danville, PA 17822
>phone 570-214-9634
>fax 570-271-5916
>
>
>IMPORTANT WARNING: The information in this message (and the documents
>attached to it, if any) is confidential and may be legally privileged. It
>is intended solely for the addressee. Access to this message by anyone
>else is unauthorized. If you are not the intended recipient, any
>disclosure, copying, distribution or any action taken, or omitted to be
>taken, in reliance on it is prohibited and may be unlawful. If you have
>received this message in error, please delete all electronic copies of
>this message (and the documents attached to it, if any), destroy any hard
>copies you may have created and notify me immediately by replying to this
>email. Thank you.
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 5
Date: Fri, 22 Jul 2005 20:14:53 +0200
From: Pablo S?nchez Quinteiro
Subject: Re: [Histonet] Endogenous peroxidase blocking
To: histonet@lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20050722201453.007b35e0@pop.lugo.usc.es>
Content-Type: text/plain; charset="us-ascii"
Dear histonetters,
Thanks a lot for all your tips!
One further question. When doing a control immunohistochemical procedure
omitting the primary antibody I guess that if I have not blocked the
endogenous peroxidase activity properly I should get staining. Isnt'it? If
in case I do not get any stain may I understand that the endogenous
activity is not a real problem?
I am working with free floating sections, polyclonal antibody, ABC, DAB.
Thanks once and again
Pablo
------------------------------
Message: 6
Date: Fri, 22 Jul 2005 13:22:49 -0500
From: "Nava, Josefa"
Subject: [Histonet] Feedback on Fc Blocker and Background buster for
Ventana IHC Machines
To:
Message-ID:
<2C515C1049EAF5459EFD8C9B929078A41944D5@phdex03.txhealth.org>
Content-Type: text/plain; charset="us-ascii"
Hello Everyone,
Can someone give me the protocol of the FC Blocker and
Background Buster reagents if you are using them for your
Ventana IHC Machines. Please give me your feedback about these 2
reagents on your Ventana Machines. Thank you.
Josie Nava
Presbyterian Hospital of Dallas
The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system.
------------------------------
Message: 7
Date: Fri, 22 Jul 2005 11:28:48 -0700
From: "K.Bowden"
Subject: Re: [Histonet] specimen database
To: "Bonner, Janet"
Cc: "'histonet@lists.utsouthwestern.edu '"
,
"'histonet-bounces@lists.utsouthwestern.edu '"
, 'Janella Seaton '
Message-ID: <42E13AE0.4040304@ucsd.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
I made a database using FileMaker Pro. It was fairly easy to create and
it is totally custom.
--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655
CONFIDENTIALITY NOTICE:
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AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR
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>Hello!
>We want to implement a database for specimens we receive in our lab.
>We would use this database starting with logging in specimens and ending
>with archiving of specimens. It would be used to track when various
>steps
>(gross photos, processing, staining, etc) have occurred and by whom.
>We do want a system that is user friendly.
>Any suggestions or advice?
>Thanks for the help.....
>j.s.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
------------------------------
Message: 8
Date: Fri, 22 Jul 2005 20:34:30 +0200
From: "Gudrun Lang"
Subject: AW: [Histonet] 2"x3" slides
To: "Histonetliste \(Histonetliste\)"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Sakura sells special forms to put in their usual racks for the bigger
slides.
Gudrun Lang
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela
Bitting
Gesendet: Freitag, 22. Juli 2005 19:00
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] 2"x3" slides
Does anyone out there have a good way to stain 2"x3" glass slides
(whole-mount prostates) using a Sakura stainer? I have a 2000 and 601
model and have tried adapting the rack so I can stain 4 slides at a
time, but it's so inefficient. Any ideas?
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Fri, 22 Jul 2005 13:35:08 -0500
From: "Nava, Josefa"
Subject: [Histonet] background on the negative controls
To:
Message-ID:
<2C515C1049EAF5459EFD8C9B929078A41944D6@phdex03.txhealth.org>
Content-Type: text/plain; charset="us-ascii"
Hello,
Has anyone had a problem on their negative controls having heavy
brown staining that looks like real DAB staining on negative mouse and
Rabbit controls that were retirieved using heat but the negative
control that used only protease treatment was very clean. I did a lot
of IHCs on adrenal mass tissue and my Rabbit and Mouse negatives look
like they have positive staining. H2O2 treatment did not correct the
problem, I also used the Avidin Biotin block. Anymore suggestions?
Thank you.
Josie
The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system.
------------------------------
Message: 10
Date: Fri, 22 Jul 2005 13:41:54 -0500
From: "LINDA MARGRAF"
Subject: [Histonet] Free Histology Learning Resources
To:
Cc: doug@visualhistology.com
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Dear Histonetters:
I got an email from the marketing director for Visual Histology and he wanted to know if it was ok to put a message out on Histonet about it. I thought it looked like something interesting so here are his comments.....
I want to make you aware of a relatively new website that provides some valuable free resources for histology teachers and students. The website is
www.VisualHistology.com and anyone who registers with the site can download
the Visual Histology Atlas (a 285 page comprehensive histology textbook from
Moran and Rowley) and also get free access to a 30-minute video tutorial
called "The Cell." The site also sells access to the complete Visual
Histology video tutorial series (26 titles) through a premium membership.
To learn more, visit: www.VisualHistology.com
If you have questions please inquire at the website as I haven't had any personal experience with these products.
Thanks
Linda M
Histonet administrator
------------------------------
Message: 11
Date: Fri, 22 Jul 2005 18:55:50 GMT
From: "mprice26@juno.com"
Subject: [Histonet] RE: Limit on amount of formalin you can pour down
sink?
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050722.115646.4572.95444@webmail28.nyc.untd.com>
Content-Type: text/plain
Hi Histonetters,
Does anyone know of any regulations as to the amount of formalin one can pour down the sink? I am in Texas.
Thank you.
Marsha Price
------------------------------
Message: 12
Date: Fri, 22 Jul 2005 14:08:06 -0500
From: "Jackie M O'Connor"
Subject: Re: [Histonet] RE: Limit on amount of formalin you can pour
down sink?
To: "mprice26@juno.com"
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Yeah - nothing. It's a hazardous chemical. I don't know of any state
where local regulations allow formalin in the wastewater.
Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
Discovery Cancer R4N2 AP3
847-938-4919
Jackie.O'Connor@abbott.com
"mprice26@juno.com"
Subject: [Histonet] CD 31
To:
Message-ID: <20050722190950.UPKM23081.ibm59aec.bellsouth.net@D6GV9G51>
Content-Type: text/plain; charset="us-ascii"
I am having a problem getting my CD31 to work. The protocol was for an
overnight incubation and pepsin pretreatment. I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab. Does anyone have any ideas? This seemed to
work well for a while but once again, I'm having problems with it. Any
input would be appreciated. Thanks in advance!
Kim Allred
Dermatopathology Associates
Jackson, MS
------------------------------
Message: 14
Date: Fri, 22 Jul 2005 15:18:24 -0400
From:
Subject: RE: [Histonet] CD 31
To: "'Kim Allred'" ,
Message-ID: <000701c58ef2$21f48530$0301a8c0@RENAD4YK9B8ABE>
Content-Type: text/plain; charset="us-ascii"
Hi Kim,
We use CD31 at 1:50, pretreatment HIER using Citrate buffer
Rena Fail
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim
Allred
Sent: Friday, July 22, 2005 3:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD 31
I am having a problem getting my CD31 to work. The protocol was for an
overnight incubation and pepsin pretreatment. I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab. Does anyone have any ideas? This seemed
to
work well for a while but once again, I'm having problems with it. Any
input would be appreciated. Thanks in advance!
Kim Allred
Dermatopathology Associates
Jackson, MS
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Fri, 22 Jul 2005 12:27:02 -0700 (PDT)
From: Steven Coakley
Subject: [Histonet] cryosectioning liver/sucrose
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20050722192702.5732.qmail@web90205.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Something a "tad" new for me here. I've never sectioned 4%Para liver infiltrated with 30% sucrose. They sectioned well but the slide appear to be loaded with what appears to be folds throughout. Not what I routinely see on regular unfixed FS. Is this normal for fixed sucrose infiltrated sections. Are there any special techniques when dealing with this special tissue.
Steve
---------------------------------
Start your day with Yahoo! - make it your home page
------------------------------
Message: 16
Date: Fri, 2 Jan 2004 09:55:02 -0900
From: "Mark Tarango"
Subject: [Histonet] Re: Black paraffin and cassettes
To:
Message-ID: <001801c3d161$eebf6760$6401a8c0@TARANGO>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I'm wondering how anyone could embed in a black tar kind of paraffin. I'm
guessing that this researcher doesn't have much experience in histology.
How would this help them?
Mark Tarango
> Date: Thu, 21 Jul 2005 19:40:26 -0500
> From: "Steven P Postl"
> Subject: [Histonet] Black paraffin and cassettes
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>
>
> Content-Type: text/plain; charset="us-ascii"
>
> A researcher is wondering (now so am I [I never heard this request
> before]) if any company sells black paraffin and black embedding
> cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
> be possible to add something to paraffin to make it stay a black color and
> remain strong as our commercial paraffin products? Thanks.
------------------------------
Message: 17
Date: Fri, 22 Jul 2005 15:36:27 -0400
From: "Bonner, Janet"
Subject: RE: [Histonet] RE: Limit on amount of formalin you can pour
down sink?
To: "'mprice26@juno.com'" ,
histonet@lists.utsouthwestern.edu
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4339@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
Orlando Florida allows it. A few of the large cities are equipped to
handle formalin in the system. You have to contact the municipal water
system people to see if they are set up to handle the formalin and receive
written confirmation for your records (and the inspectors) before you start
dumping ANY of it in the sink.
We just started recycling ours with a Creative Waste Solutions,Inc
Formalin recycler (rex@cwsincorp.com) and have been very pleased with the
results. How and why it works I leave to him to explain, but it does.
Janet
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
mprice26@juno.com
Sent: Friday, July 22, 2005 2:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Limit on amount of formalin you can pour down
sink?
Hi Histonetters,
Does anyone know of any regulations as to the amount of formalin one can
pour down the sink? I am in Texas.
Thank you.
Marsha Price
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Fri, 22 Jul 2005 16:17:52 -0400
From: "Monfils, Paul"
Subject: RE: [Histonet] cryosectioning liver/sucrose
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE01717588@lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
At any given temperature, tissues infiltrated with sucrose tend to be softer
and less completely frozen than similar tissues without sucrose (and 30% is
a very high level of sucrose), and therefore they compress and wrinkle more.
Try cutting them at a colder temperature than you would normally use for
liver. That will probably help produce flatter sections with less
wrinkling.
> ----------
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of
> Steven Coakley
> Sent: Friday, July 22, 2005 12:27 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryosectioning liver/sucrose
>
> Something a "tad" new for me here. I've never sectioned 4%Para liver
> infiltrated with 30% sucrose. They sectioned well but the slide appear to
> be loaded with what appears to be folds throughout. Not what I routinely
> see on regular unfixed FS. Is this normal for fixed sucrose infiltrated
> sections. Are there any special techniques when dealing with this special
> tissue.
>
> Steve
>
>
>
> ---------------------------------
> Start your day with Yahoo! - make it your home page
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 19
Date: Fri, 22 Jul 2005 14:44:04 -0700
From: "Trisha Emry"
Subject: [Histonet] under decal
To: "histo"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Is there anything that can be done for under decalcified bone after it has
been processed?
Trisha
Seattle
------------------------------
Message: 20
Date: Fri, 22 Jul 2005 18:13:21 -0400
From: "Bonner, Janet"
Subject: RE: [Histonet] under decal
To: "'Trisha Emry'" , "histo"
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB433B@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
OPTION 1: "redecal" Melt the paraffin block, put the tissue in a cassette
as you would a fresh piece of tissue to process. Drop it in xylene for 15
minutes x2, then 100% abs. alcohol x2, 95% abs. alcohol. Wash in water and
drop back into the decal. Process when soft.
OPTION 2: "surface decal" Pour some decal solution into a small container
or Petri dish and put the faced block face-down in the solution. Let soak
for 15 min (time depends on hardness of the tissue) put on ice and try to
section. Only the surface will be decaled so try to take a section off the
surface.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Trisha
Emry
Sent: Friday, July 22, 2005 5:44 PM
To: histo
Subject: [Histonet] under decal
Is there anything that can be done for under decalcified bone after it has
been processed?
Trisha
Seattle
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Fri, 22 Jul 2005 18:23:51 -0400
From: "Monfils, Paul"
Subject: RE: [Histonet] under decal
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE01717589@lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
Sometimes you can get a decent section by pressing gauze soaked with decal
fluid on the face of the block for a couple of minutes (wear gloves), then
taking the first section off the face. Or you may get a few successive
sections by soaking the faced block in decal fluid for 15 to 30 minutes,
then chilling the block on the cold tray and cutting right off the face.
However, if you are going to be doing any substantial amount of work with
that block, probably the best approach is to reverse process it, decal it
properly, and reprocess it. Remove the paraffin by soaking in xylene or
whatever clearing agent you use, remove the xylene with absolute alcohol,
bring the specimen down to water, then decal it, wash it and process it as
usual. If you want you can place it in formalin overnight once it reaches
the water stage, to ensure the best possible fixation before decalcifying
it. Since most tissue processors won't run the stations in reverse order,
you'll probably have to do the reverse processing by hand, in a beaker or
flask. If the tissue was well fixed initially, it should withstand this
treatment with little if any noticeable harm.
Paul M.
> ----------
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of
> Trisha Emry
> Sent: Friday, July 22, 2005 2:44 PM
> To: histo
> Subject: [Histonet] under decal
>
> Is there anything that can be done for under decalcified bone after it has
> been processed?
>
> Trisha
> Seattle
>
>
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 22
Date: Fri, 22 Jul 2005 15:48:27 -0700
From: Lori Richey
Subject: Re: [Histonet] CD 31
To: Kim Allred
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <42E177BB.2050905@u.washington.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
We use DAKO CD31 1:25 , 18 minutes microwave, in citrate buffer.
Kim Allred wrote:
>I am having a problem getting my CD31 to work. The protocol was for an
>overnight incubation and pepsin pretreatment. I couldn't get it to work
>this way so I altered it a bit by using trilogy for AR and a two hour
>incubation for the primary ab. Does anyone have any ideas? This seemed to
>work well for a while but once again, I'm having problems with it. Any
>input would be appreciated. Thanks in advance!
>
>
>
>
>
>
>
>Kim Allred
>
>Dermatopathology Associates
>
>Jackson, MS
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 23
Date: Sat, 23 Jul 2005 04:19:18 EDT
From: IamNinaOwen@aol.com
Subject: [Histonet] Staining for Helicobacter pylori
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Hi everyone!
I'm doing a project on different staining methods for H. pylori in gastric
biopsies, and just out of interest really wondered which special stains
different labs routinely use for this purpose. My lab uses the Modified Giemsa.
Thanks
Nina Owen
------------------------------
Message: 24
Date: Sat, 23 Jul 2005 11:39:27 -0400
From: Drew Meyer <41dmb41@gmail.com>
Subject: Re: [Histonet] Staining for Helicobacter pylori
To: "IamNinaOwen@aol.com"
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=ISO-8859-1
We use the Wharton Stary method.... you can also use an Alcian Yellow
Stain... there's another called the Toluidine Method I believe...
Hope this helps...
Drew Meyer
On 7/23/05, IamNinaOwen@aol.com wrote:
> Hi everyone!
>
> I'm doing a project on different staining methods for H. pylori in gastric
> biopsies, and just out of interest really wondered which special stains
> different labs routinely use for this purpose. My lab uses the Modified Giemsa.
>
> Thanks
>
> Nina Owen
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
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End of Histonet Digest, Vol 20, Issue 31
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