[Histonet] TMB staining for WGA-HRP


We are about to cut a brain that was injected with
WGA-HRP for tract tracing. We haven't done this
for quite a while (decades?) and don't remember
if it is possible to delay processing after cutting.
If it is possible, how long can we delay, and what
solution should the cut tissue be stored in?
We plan to stain using the Olucha molybybdate
modification to the Mesulam TMB procedure.

Bob Nienhuis
UCLA / VA Medical Center
Los Angeles, CA

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