[Histonet] Rat brains

From:Laura Fidgen

Please help...
A research project for our neuropathologist includes rat brains.  These are 
extremely difficult to section as he profuses the whole rat with 
glutaraldehyde then takes brain samples.  I have tried adding alcohol to my 
water bath but these sections still have many, many wrinkles.  Can anyone 
offer suggestions?  I would be so grateful for any and all advice.

Thank You!!

Laura L. Fidgen, MScF, BSc, HT(ASCP)
Laboratory and Research Practioner
VMRCVM, Histopatholgy
Blacksburg, VA 24060-0443 


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