[Histonet] RE: Histonet Digest, Vol 20, Issue 21

From:"Goodwin, Diana"

At our institution, the Anatomic Path dept. is responsible for "morgue
patient inventory" and releasing bodies.  This has been a point of
contention for years, ever since they eliminated the position of "morgue
attendant", but one that our administration insists is the pathology dept's
responsibility.  

At the institution where I previously worked, body release was handled by
the security dept, with the central switchboard in control of the status of
decedents in the hospital via the HIS.

Good luck!

Diana G. Goodwin
Department of Pathology
Pennsylvania Hospital
800 Spruce St., Preston 655-C
Philadelphia, PA  19107
 
ph:  215-829-6532
fax:  215-829-7564
e-mail:  goodwind@pahosp.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]
Sent: Monday, July 18, 2005 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 21


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Today's Topics:

   1. RE: dissecting board (Malam Jacqueline)
   2. Need help with PGP9.5 on FFPE skin  (Nicola Cragg)
   3. RE: automated microtomes (Bonner, Janet)
   4. Re: Dissection boards (Fred Underwood)
   5. Bodies in morgue - who does the checking? (Paula Wilder)


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Message: 1
Date: Mon, 18 Jul 2005 12:39:23 +0100
From: Malam Jacqueline 
Subject: [Histonet] RE: dissecting board
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: 
Content-Type: text/plain

Thermo Shandon do 2 colours and 2 sizes of polyethylene board.
They're easy to clean and last. We actually got ours from a butchers'
suppliers!

-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu] 
Sent: 17 July 2005 18:07
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 19

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When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. Dissection boards (Katia Cristina Catunda)


----------------------------------------------------------------------

Message: 1
Date: Sat, 16 Jul 2005 22:20:15 -0300
From: "Katia Cristina Catunda" 
Subject: [Histonet] Dissection boards
To: 
Message-ID: <019801c58a6d$b0bbc050$a279fea9@privatexx>
Content-Type: text/plain;	charset="iso-8859-1"

In our lab we still use wood-dissecting boards but we really want to change
it!! (wood can be very very very dirty after some years even if we submit
the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and what
is the best option, buy it from distributors like Mopec or to pay for
someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia

------------------------------

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End of Histonet Digest, Vol 20, Issue 19
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Message: 2
Date: Mon, 18 Jul 2005 13:39:29 +0100
From: "Nicola Cragg" 
Subject: [Histonet] Need help with PGP9.5 on FFPE skin 
To: 
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Hello,

I'm posting this message with some embarrassment and apologise for raising
anybody's hope of a quck solution........
I have been trying to optimise PGP9.5 on FFPE skin samples (3 micron), using
a mouse monoclonal (clone 10A1) from Neuromics.  Despite my initial
excitement expressed in an earlier posting, I have since found some very
good photos of PGP9.5 staining in published work which has put mine to shame
and has made me realise that my IHC is not working well enough at all.  I've
found an improvement with the waterbath antigen retrieval, as the dermis
remains intact and there is some specific  staining (I think) in the
reticular dermis, which wasn't apparent with microwave antigen retrieval.  

However, it appears that there seems to be more of a trend of using
free-floating 50 micron sections.  Is that because routine FFPE sections are
difficult to stain for this antigen?  Has anyone got it to work on FFPE
samples?  I was quite hopeful that it would work from reading the datasheet
and I have also found that Dako make a Rabbit polyclonal for FFPE sections
and they're usually excellent antibodies so I'm hoping to try.  Any advice
or suggestions will be gratefully received.

Regards,

Nicola Cragg


------------------------------

Message: 3
Date: Mon, 18 Jul 2005 08:43:37 -0400
From: "Bonner, Janet" 
Subject: RE: [Histonet] automated microtomes
To: "'Sennello, Gina '" ,
	"'histonet-bounces@lists.utsouthwestern.edu '"
	,	"'Histonet Histonet
	(E-mail) '" 
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4325@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1

 We have used the Leica's with great success and few if any call backs after
more than eight years!!    Janet

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
To: Histonet Histonet (E-mail)
Sent: 7/13/2005 3:12 PM
Subject: [Histonet] automated microtomes



I am in the market for  fully automated microtome and have looked at
Leica's RM2255, Thermo-Electron's Finesse ME and Microm's 355S.  Does
anyone
have any strong feels for against any of these instruments?

Thanks in advance for you opinions and help.

Gina

Gina Sennello
Senior Associate Scientist
Histotechnologist

OSIP
2860 Wilderness Place
Boulder, CO
80301

phone 303-546-7739
fax      303-444-0672


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------------------------------

Message: 4
Date: Mon, 18 Jul 2005 12:29:54 -0400
From: "Fred Underwood" 
Subject: Re: [Histonet] Dissection boards
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I've found that kitchen cutting boards from your local department store
works well, and it's cheaper than from a medical supplier.

Fred

>>> "Katia Cristina Catunda"  07/16/05 09:20PM
>>>
In our lab we still use wood-dissecting boards but we really want to
change it!! (wood can be very very very dirty after some years even if
we submit the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and
what is the best option, buy it from distributors like Mopec or to pay
for someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia
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Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Mon, 18 Jul 2005 16:37:34 +0000
From: "Paula Wilder" 
Subject: [Histonet] Bodies in morgue - who does the checking?
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue at 
your respective instituition would be greatly appreciated.  So far, by 
phoning neighboring hospitals, I have found that Security, a diener service,

or the Pathology Assistants are the ones responsible.  Any help in this 
would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741





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