[Histonet] RE: Confusing IHC results
Dusco,
Patty is completely right about this! I have observed the same problem
with FFPE kidney sections that were pronase pretreated.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Wed, 20 Jul 2005 16:33:04 -0600
From: "Patsy Ruegg"
Subject: RE: [Histonet] Confusing IHC results
To: "'Trajkovic, Dusko'" ,
In my experience the endogenous biotin in liver cannot be completely
removed
even with the most aggressive AB blocking, this is why I do not use an
avidin/biotin detection system, I use labeled polymer instead.
Patsy
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