Dusco,

   Patty is completely right about this! I have observed the same problem
   with FFPE kidney sections that were pronase pretreated.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Wed, 20 Jul 2005 16:33:04 -0600
   From: "Patsy Ruegg" 
   Subject: RE: [Histonet] Confusing IHC results
   To: "'Trajkovic, Dusko'" ,
   
   In  my  experience the endogenous biotin in liver cannot be completely
   removed
   even with the most aggressive AB blocking, this is why I do not use an
   avidin/biotin detection system, I use labeled polymer instead.
   Patsy
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