[Histonet] Problems of processing brain for paraffin embedding
|From:||azita parvaneh tafreshi |
I have problems with paraffin processing of mouse and hamster brains and spinal cords. I have perfused the animals with PFA 4% and I postfixed them in the same buffer for 7-8 days and then stored in Alc 70% (up to now). After sectioning, tissues wrinkle in a way that it is torn after flattenning (on a slide warmer), therefore the quality is not good at all.
Can anyone give me hints to overcome the problem. A detailted protocol of paraffin processing would be great.
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