[Histonet] Hema tek wright giemsa stainer calling vendors!

From:"Madary, Joseph"

Any vendor or anyone out there that can get me a quote on a wright giemsa stainer, we are in the market.  Specifically the Hema Tek 2000 unless someone knows of a better one.  I am in the DC MD area.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, July 19, 2005 11:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 22


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Today's Topics:

   1. RE: Bodies in morgue - who does the checking? (Charles.Embrey)
   2. How is everyone carrying out the new CAP req.? Is there
      documented evidence of daily review of the (Billie Zimmerman)
   3. RE: Bodies in morgue - who does the checking? (Horn, Hazel V)
   4. liquid nitrogen (manal galal)
   5. RE: How is everyone carrying out the new CAP req.? Is
      theredocumented evidence of daily review of the (Sebree Linda A.)
   6. CAP inspections (Histology SLU)
   7. seeking part time position (Goodwin, Diana)
   8. How is everyone carrying.... (DMBCMP@aol.com)
   9. Workloads vs Hiso staffing (Maray Weirauch)
  10. Re: mouse brain (Geoff McAuliffe)
  11. RE: Histonet Digest, Vol 20, Issue 21 (Goodwin, Diana)
  12. PGP9.5 (Dodson, Cecelia)
  13. Re: liquid nitrogen (Karen  Weidenheim)
  14. RE: liquid nitrogen (Smith, Allen)
  15. Re: CAP inspections (Joe Nocito)
  16. Re: How is everyone carrying out the new CAP req.? Is
      theredocumented evidence of daily review of the (Joe Nocito)
  17. RE: Bodies in morgue - who does the checking?
      (Rogerson Kemlo (ELHT) Pathology)
  18. unsubscribe (Dunn-Jena, Patsy A)
  19. Cytology Immuno's (Jean Gillson)
  20. Problem with Eosin Y staining (Habitzruther, Michael)
  21. RE: Problem with Eosin Y staining
      (Rogerson Kemlo (ELHT) Pathology)


----------------------------------------------------------------------

Message: 1
Date: Mon, 18 Jul 2005 12:01:38 -0500
From: "Charles.Embrey" 
Subject: RE: [Histonet] Bodies in morgue - who does the checking?
To: "Paula Wilder" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

It depends what you mean.  Check bodies into the morgue or check bodies
that are in the morgue.  Here we have a house officer (nurse) that
coordinates the release of bodies from the hospital through security.
Sometimes if the funeral home can come quickly enough then they take
them from the hospital room. If they can't come quickly the ward staff
put the body into the morgue with the help of security.  I keep an eye
on who is in my morgue so I can get a jump on any autopsy that might be
required.  Since I do all the weekday autopsies I don't like surprises.
My dieners are all part-time (as needed) and my pathology assistant is
only concerned with setting up gross and logging specimens into the
computer (I can't get her to go near the morgue).  I did work at one
place in Ohio where the diener was full time and actually had his office
in the morgue.  Hope this helps....

Charles Embrey, PA(ASCP)
Pathologists' Assistant
Histology Manager   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula
Wilder
Sent: Monday, July 18, 2005 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bodies in morgue - who does the checking?

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue
at 
your respective instituition would be greatly appreciated.  So far, by 
phoning neighboring hospitals, I have found that Security, a diener
service, 
or the Pathology Assistants are the ones responsible.  Any help in this 
would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741



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Histonet mailing list
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------------------------------

Message: 2
Date: Mon, 18 Jul 2005 13:09:53 -0400
From: "Billie Zimmerman" 
Subject: [Histonet] How is everyone carrying out the new CAP req.? Is
	there	documented evidence of daily review of the
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

How is everyone carrying out the new CAP req.?   Is there documented
evidence of daily review of the technical quality of histologic
preparations by the pathologist?

This req. came out in April so I was wondering the best way to handle
this.

Thanks,
Billie Zimmerman
Medical College of GA





------------------------------

Message: 3
Date: Mon, 18 Jul 2005 12:13:02 -0500
From: "Horn, Hazel V" 
Subject: RE: [Histonet] Bodies in morgue - who does the checking?
To: "Charles.Embrey" ,	"Paula Wilder"
	, histonet@lists.utsouthwestern.edu
Message-ID:
	<9AE8AA9E1F644B4AA6C155FB6FD51C63038BDDA4@EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii

At our hospital is is histology's responsibility to check the morgue on
day shift, Monday-Friday.    On evenings, nights and weekends our
Nursing service does it.   BUT we can check the morgue, via computer.
We have the option to pull up and expired patient list.  If we know
someone has expired we can go down to the morgue and check to see if
there is autopsy.    We also release bodies on dayshift to the funeral
homes.     


Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital - Changing Children's Lives

Phone - 501.364.4240
Fax - 501.364.3912 

Visit us on the web at      www. archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Charles.Embrey
Sent: Monday, July 18, 2005 12:02 PM
To: Paula Wilder; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Bodies in morgue - who does the checking?

It depends what you mean.  Check bodies into the morgue or check bodies
that are in the morgue.  Here we have a house officer (nurse) that
coordinates the release of bodies from the hospital through security.
Sometimes if the funeral home can come quickly enough then they take
them from the hospital room. If they can't come quickly the ward staff
put the body into the morgue with the help of security.  I keep an eye
on who is in my morgue so I can get a jump on any autopsy that might be
required.  Since I do all the weekday autopsies I don't like surprises.
My dieners are all part-time (as needed) and my pathology assistant is
only concerned with setting up gross and logging specimens into the
computer (I can't get her to go near the morgue).  I did work at one
place in Ohio where the diener was full time and actually had his office
in the morgue.  Hope this helps....

Charles Embrey, PA(ASCP)
Pathologists' Assistant
Histology Manager   

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula
Wilder
Sent: Monday, July 18, 2005 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bodies in morgue - who does the checking?

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue
at your respective instituition would be greatly appreciated.  So far,
by phoning neighboring hospitals, I have found that Security, a diener
service, or the Pathology Assistants are the ones responsible.  Any help
in this would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 4
Date: Sat, 16 Jul 2005 05:06:46 -0700 (PDT)
From: manal galal 
Subject: [Histonet] liquid nitrogen
To: Histonet@Pathology.swmed.edu
Message-ID: <20050716120646.93890.qmail@web50204.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi all, 
 
  I wanted to ask about how to use liquid nitogen in freezing muscle biopsies. I mean what does it come in? How much do I use? Do I discard it after I use it? What kind of container do I use? How long do I put the biopsy in it? How do I store it? 
  By the way I freeze muscle in the quick freezing chamber of the cryostat, and am getting very little to no artifacts. But anyone that learns of how I freeeze biopsies says that it will give me disasterous resuls. Do you think liquid nitrogen will be superior to my old method.
 
Thanks in advance
manal

__________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
http://mail.yahoo.com 

------------------------------

Message: 5
Date: Mon, 18 Jul 2005 12:36:12 -0500
From: "Sebree Linda A." 
Subject: RE: [Histonet] How is everyone carrying out the new CAP req.?
	Is	theredocumented evidence of daily review of the
To: "Billie Zimmerman" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

As technologists, we sign off on the run sheets of each instrument batch
of slides.  This indicates that the positive and negative controls have
stained as expected and that the patient unknowns are of high quality.
If something is not as it should be at this point, we may decide on our
own to rerun a case or slides and inform the pathologist or we discuss
it with the requesting pathologist and come up with a plan of action,
which is documented on the run sheet.  When the slides are delivered to
the pathologists, a QA form accompanies them and the pathologist is
required to indicate "satisfactory" or "unsatisfactory" and sign his
name.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Billie
Zimmerman
Sent: Monday, July 18, 2005 12:10 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] How is everyone carrying out the new CAP req.? Is
theredocumented evidence of daily review of the


How is everyone carrying out the new CAP req.?   Is there documented
evidence of daily review of the technical quality of histologic
preparations by the pathologist?

This req. came out in April so I was wondering the best way to handle
this.

Thanks,
Billie Zimmerman
Medical College of GA



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 6
Date: Mon, 18 Jul 2005 10:38:19 -0700 (PDT)
From: Histology SLU 
Subject: [Histonet] CAP inspections
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050718173819.2450.qmail@web51011.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello All:
 
This is directed at those of you that may have had your CAP inspection in the last year.  I have been hearing that the inspectors have been, shall we say, interpreting some of the checklist questions in an unusual manner.  My question to you all is, were there any questions where you got "tagged" for something that you had always done and had passed previously?  Also, can you share ANY experiences that you had through your CAP inspection good or bad?  As always, thank you for your insights and opinions.  This is a super group and I always appreciate your comments.
 
Susan

__________________________________________________
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Tired of spam?  Yahoo! Mail has the best spam protection around 
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------------------------------

Message: 7
Date: Mon, 18 Jul 2005 11:56:14 -0400
From: "Goodwin, Diana" 
Subject: [Histonet] seeking part time position
To: "Histonet (E-mail)" 
Message-ID:
	<992899E9EC268548AB8DDE246AF88473055F5266@PAHEX01.uphs.upenn.edu>
Content-Type: text/plain;	charset="iso-8859-1"

 
Experienced histo tech seeking part time position in Philadelphia area.  If
interested, contact Coleen Kiehl at 215-829-6940.
 


------------------------------

Message: 8
Date: Mon, 18 Jul 2005 14:30:48 EDT
From: DMBCMP@aol.com
Subject: [Histonet] How is everyone carrying....
To: Histonet@lists.utsouthwestern.edu
Message-ID: <129.6119d036.300d4f58@aol.com>
Content-Type: text/plain; charset="US-ASCII"

Hi:
Just wanted to ad that we, also, send a QC sheet to each of our pathologists 
every morning with their first run of slides.  They respond on the sheet, even 
though they speak to us about any problem, of course. This is a way for us to 
keep a record for CAP. 

Dannie Blake, HT
Community Medical Centers
Fresno, Ca



------------------------------

Message: 9
Date: Mon, 18 Jul 2005 14:44:41 -0400
From: "Maray Weirauch" 
Subject: [Histonet] Workloads vs Hiso staffing
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

** PRIVATE **

Our hospital is conducting an analysis of laboratory workload, workflow
and staffing.  This will include Histology as well, and we are being
asked for input with any national benchmarks available for surgical
histology tasks (i.e. average number cases accessioned, blocks embedded,
slides cut../some type of time unit)  Does anyone know of some realistic
published values or ranges?  Thanks!



------------------------------

Message: 10
Date: Mon, 18 Jul 2005 14:42:07 -0400
From: Geoff McAuliffe 
Subject: Re: [Histonet] mouse brain
To: "Atoska S. Gentry" 
Cc: Histonet 
Message-ID: <42DBF7FF.8080406@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Hi Atoska:

    Hydrocephalus is not uncommon in mice, perhaps that is the source of 
your concern? I suppose it could be manifested more on one side than the 
other? Maybe you could post a photo?

Geoff

Atoska S. Gentry wrote:

>  Hello, please have any of you who process & section paraformaldehyde 
> fixed, paraffin embedded mouse brain experienced asymmetry of the 
> right & left hemispheres? Recently our coronal mouse brain sections 
> which appear symmetrical upon gross trim and after initial facing of 
> paraffin are asymmetrical after staining. I've rotated the block 
> holder to the limit and sectioned the most rostral hemisphere at 
> higher micron thickness to accommodate. However, to my dismay and 
> disappointment none of this has helped very much. Any pointers in 
> remedying this situation ASAP will be greatly appreciated. Thanks, Atoska
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
**********************************************





------------------------------

Message: 11
Date: Mon, 18 Jul 2005 15:27:58 -0400
From: "Goodwin, Diana" 
Subject: [Histonet] RE: Histonet Digest, Vol 20, Issue 21
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<992899E9EC268548AB8DDE246AF88473055F526B@PAHEX01.uphs.upenn.edu>
Content-Type: text/plain;	charset="iso-8859-1"

At our institution, the Anatomic Path dept. is responsible for "morgue
patient inventory" and releasing bodies.  This has been a point of
contention for years, ever since they eliminated the position of "morgue
attendant", but one that our administration insists is the pathology dept's
responsibility.  

At the institution where I previously worked, body release was handled by
the security dept, with the central switchboard in control of the status of
decedents in the hospital via the HIS.

Good luck!

Diana G. Goodwin
Department of Pathology
Pennsylvania Hospital
800 Spruce St., Preston 655-C
Philadelphia, PA  19107
 
ph:  215-829-6532
fax:  215-829-7564
e-mail:  goodwind@pahosp.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]
Sent: Monday, July 18, 2005 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 21


Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: dissecting board (Malam Jacqueline)
   2. Need help with PGP9.5 on FFPE skin  (Nicola Cragg)
   3. RE: automated microtomes (Bonner, Janet)
   4. Re: Dissection boards (Fred Underwood)
   5. Bodies in morgue - who does the checking? (Paula Wilder)


----------------------------------------------------------------------

Message: 1
Date: Mon, 18 Jul 2005 12:39:23 +0100
From: Malam Jacqueline 
Subject: [Histonet] RE: dissecting board
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: 
Content-Type: text/plain

Thermo Shandon do 2 colours and 2 sizes of polyethylene board.
They're easy to clean and last. We actually got ours from a butchers'
suppliers!

-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu] 
Sent: 17 July 2005 18:07
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 19

Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request@lists.utsouthwestern.edu

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	histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. Dissection boards (Katia Cristina Catunda)


----------------------------------------------------------------------

Message: 1
Date: Sat, 16 Jul 2005 22:20:15 -0300
From: "Katia Cristina Catunda" 
Subject: [Histonet] Dissection boards
To: 
Message-ID: <019801c58a6d$b0bbc050$a279fea9@privatexx>
Content-Type: text/plain;	charset="iso-8859-1"

In our lab we still use wood-dissecting boards but we really want to change
it!! (wood can be very very very dirty after some years even if we submit
the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and what
is the best option, buy it from distributors like Mopec or to pay for
someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia

------------------------------

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End of Histonet Digest, Vol 20, Issue 19
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------------------------------

Message: 2
Date: Mon, 18 Jul 2005 13:39:29 +0100
From: "Nicola Cragg" 
Subject: [Histonet] Need help with PGP9.5 on FFPE skin 
To: 
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Hello,

I'm posting this message with some embarrassment and apologise for raising
anybody's hope of a quck solution........
I have been trying to optimise PGP9.5 on FFPE skin samples (3 micron), using
a mouse monoclonal (clone 10A1) from Neuromics.  Despite my initial
excitement expressed in an earlier posting, I have since found some very
good photos of PGP9.5 staining in published work which has put mine to shame
and has made me realise that my IHC is not working well enough at all.  I've
found an improvement with the waterbath antigen retrieval, as the dermis
remains intact and there is some specific  staining (I think) in the
reticular dermis, which wasn't apparent with microwave antigen retrieval.  

However, it appears that there seems to be more of a trend of using
free-floating 50 micron sections.  Is that because routine FFPE sections are
difficult to stain for this antigen?  Has anyone got it to work on FFPE
samples?  I was quite hopeful that it would work from reading the datasheet
and I have also found that Dako make a Rabbit polyclonal for FFPE sections
and they're usually excellent antibodies so I'm hoping to try.  Any advice
or suggestions will be gratefully received.

Regards,

Nicola Cragg


------------------------------

Message: 3
Date: Mon, 18 Jul 2005 08:43:37 -0400
From: "Bonner, Janet" 
Subject: RE: [Histonet] automated microtomes
To: "'Sennello, Gina '" ,
	"'histonet-bounces@lists.utsouthwestern.edu '"
	,	"'Histonet Histonet
	(E-mail) '" 
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4325@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1

 We have used the Leica's with great success and few if any call backs after
more than eight years!!    Janet

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
To: Histonet Histonet (E-mail)
Sent: 7/13/2005 3:12 PM
Subject: [Histonet] automated microtomes



I am in the market for  fully automated microtome and have looked at
Leica's RM2255, Thermo-Electron's Finesse ME and Microm's 355S.  Does
anyone
have any strong feels for against any of these instruments?

Thanks in advance for you opinions and help.

Gina

Gina Sennello
Senior Associate Scientist
Histotechnologist

OSIP
2860 Wilderness Place
Boulder, CO
80301

phone 303-546-7739
fax      303-444-0672


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------------------------------

Message: 4
Date: Mon, 18 Jul 2005 12:29:54 -0400
From: "Fred Underwood" 
Subject: Re: [Histonet] Dissection boards
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I've found that kitchen cutting boards from your local department store
works well, and it's cheaper than from a medical supplier.

Fred

>>> "Katia Cristina Catunda"  07/16/05 09:20PM
>>>
In our lab we still use wood-dissecting boards but we really want to
change it!! (wood can be very very very dirty after some years even if
we submit the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and
what is the best option, buy it from distributors like Mopec or to pay
for someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Mon, 18 Jul 2005 16:37:34 +0000
From: "Paula Wilder" 
Subject: [Histonet] Bodies in morgue - who does the checking?
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue at 
your respective instituition would be greatly appreciated.  So far, by 
phoning neighboring hospitals, I have found that Security, a diener service,

or the Pathology Assistants are the ones responsible.  Any help in this 
would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741





------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 20, Issue 21
****************************************


------------------------------

Message: 12
Date: Mon, 18 Jul 2005 15:26:05 -0500
From: "Dodson, Cecelia" 
Subject: [Histonet] PGP9.5
To: 
Message-ID:
	<6A65BA49B1BEEA419A3B4D16DFA8EC5A08283D@CHEX1.chp.clarian.org>
Content-Type: text/plain;	charset="iso-8859-1"

We are staining PGP9.5 on a regular bases using a polyclonal antibody we purchase from Biogenesis.  It is antigen retrieved in a steamer for 15 minutes then stained with LSAB2 from Dako.  Cecelia

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Monday, July 18, 2005 12:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 21


Send Histonet mailing list submissions to
	histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
	histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: dissecting board (Malam Jacqueline)
   2. Need help with PGP9.5 on FFPE skin  (Nicola Cragg)
   3. RE: automated microtomes (Bonner, Janet)
   4. Re: Dissection boards (Fred Underwood)
   5. Bodies in morgue - who does the checking? (Paula Wilder)


----------------------------------------------------------------------

Message: 1
Date: Mon, 18 Jul 2005 12:39:23 +0100
From: Malam Jacqueline 
Subject: [Histonet] RE: dissecting board
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: 
Content-Type: text/plain

Thermo Shandon do 2 colours and 2 sizes of polyethylene board.
They're easy to clean and last. We actually got ours from a butchers'
suppliers!

-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu] 
Sent: 17 July 2005 18:07
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 19

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When replying, please edit your Subject line so it is more specific than
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Today's Topics:

   1. Dissection boards (Katia Cristina Catunda)


----------------------------------------------------------------------

Message: 1
Date: Sat, 16 Jul 2005 22:20:15 -0300
From: "Katia Cristina Catunda" 
Subject: [Histonet] Dissection boards
To: 
Message-ID: <019801c58a6d$b0bbc050$a279fea9@privatexx>
Content-Type: text/plain;	charset="iso-8859-1"

In our lab we still use wood-dissecting boards but we really want to change
it!! (wood can be very very very dirty after some years even if we submit
the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and what
is the best option, buy it from distributors like Mopec or to pay for
someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia

------------------------------

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End of Histonet Digest, Vol 20, Issue 19
****************************************


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------------------------------

Message: 2
Date: Mon, 18 Jul 2005 13:39:29 +0100
From: "Nicola Cragg" 
Subject: [Histonet] Need help with PGP9.5 on FFPE skin 
To: 
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Hello,

I'm posting this message with some embarrassment and apologise for raising anybody's hope of a quck solution........
I have been trying to optimise PGP9.5 on FFPE skin samples (3 micron), using a mouse monoclonal (clone 10A1) from Neuromics.  Despite my initial excitement expressed in an earlier posting, I have since found some very good photos of PGP9.5 staining in published work which has put mine to shame and has made me realise that my IHC is not working well enough at all.  I've found an improvement with the waterbath antigen retrieval, as the dermis remains intact and there is some specific  staining (I think) in the reticular dermis, which wasn't apparent with microwave antigen retrieval.  

However, it appears that there seems to be more of a trend of using free-floating 50 micron sections.  Is that because routine FFPE sections are difficult to stain for this antigen?  Has anyone got it to work on FFPE samples?  I was quite hopeful that it would work from reading the datasheet and I have also found that Dako make a Rabbit polyclonal for FFPE sections and they're usually excellent antibodies so I'm hoping to try.  Any advice or suggestions will be gratefully received.

Regards,

Nicola Cragg


------------------------------

Message: 3
Date: Mon, 18 Jul 2005 08:43:37 -0400
From: "Bonner, Janet" 
Subject: RE: [Histonet] automated microtomes
To: "'Sennello, Gina '" ,
	"'histonet-bounces@lists.utsouthwestern.edu '"
	,	"'Histonet Histonet
	(E-mail) '" 
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4325@fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1

 We have used the Leica's with great success and few if any call backs after
more than eight years!!    Janet

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
To: Histonet Histonet (E-mail)
Sent: 7/13/2005 3:12 PM
Subject: [Histonet] automated microtomes



I am in the market for  fully automated microtome and have looked at
Leica's RM2255, Thermo-Electron's Finesse ME and Microm's 355S.  Does
anyone
have any strong feels for against any of these instruments?

Thanks in advance for you opinions and help.

Gina

Gina Sennello
Senior Associate Scientist
Histotechnologist

OSIP
2860 Wilderness Place
Boulder, CO
80301

phone 303-546-7739
fax      303-444-0672


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------------------------------

Message: 4
Date: Mon, 18 Jul 2005 12:29:54 -0400
From: "Fred Underwood" 
Subject: Re: [Histonet] Dissection boards
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I've found that kitchen cutting boards from your local department store
works well, and it's cheaper than from a medical supplier.

Fred

>>> "Katia Cristina Catunda"  07/16/05 09:20PM
>>>
In our lab we still use wood-dissecting boards but we really want to
change it!! (wood can be very very very dirty after some years even if
we submit the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and
what is the best option, buy it from distributors like Mopec or to pay
for someone to make them?
Some simple questions that makes a lot of difference for us...

Thanks

Katia
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Mon, 18 Jul 2005 16:37:34 +0000
From: "Paula Wilder" 
Subject: [Histonet] Bodies in morgue - who does the checking?
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue at 
your respective instituition would be greatly appreciated.  So far, by 
phoning neighboring hospitals, I have found that Security, a diener service, 
or the Pathology Assistants are the ones responsible.  Any help in this 
would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741





------------------------------

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End of Histonet Digest, Vol 20, Issue 21
****************************************



------------------------------

Message: 13
Date: Mon, 18 Jul 2005 16:51:26 -0400
From: "Karen  Weidenheim" 
Subject: Re: [Histonet] liquid nitrogen
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Hi Manal, 
The group has been discussing this topic lately.  Our lab does
classical muscle biopsy freezing with liquid nitrogen and isopentane. 
(I am happy to hear that you are successful with the cryostat and will
have to check this out, but anyway, here is a little synopsis):
1.  Liquid nitrogen comes in a large pressurized tank that one orders
from a company that provides compressed gases.
2.  For freezing,  We fill a 2 liter Dewar flask , Cat # 2119, Lab Line
Instruments Inc, Melrose Park, IL about 75% full of liquid nitrogen and
we suspend our 250 ml stainless steel, very clean beaker that is around
65% full of isopentane in it using a home-made wire contraption made
from coat hangers (!) that encircles the beaker and allows us to lower
it slowly in to the isopentane (this whole procedure requires 2 people,
1 to handle the isopentane and 1 to do the specimen.)
If you are alone, say, on call at night, you can use a ring stand to
hold the beaker suspended by the wires.  Or you can bend the wire
contraption so it hooks on the side of the beaker.  
When ordering your equipment, you have to be sure, by measuring the
internal diameter of the Dewar flask and the external diameter of your
stainless beaker, that you can suspend the beaker in the flask
comfortably.  You have to lower the beaker of isopentane slowly into the
liquid nitrogen to avoid the liquid nitrogen boiling into the isopentane
and making a mess, at which point you have to start over.
3.  We freeze a 0.8 x 0.5 cm portion of oriented skeletal muscle 25
seconds in syrupy isopentane that has ice all around the sides and the
bottom.  THe textbooks say 5-10 seconds, but we find we need 25 seconds.
 I think lots of this method is empiric and depends on your location,
altitude and humidity.  
We never reuse the isopentane which, by the way, has to be kept in a
flammable cabinet according to regulations.  I might do 4 blocks in one
aliquot of isopentane but I change it frequently and I think this helps
me avoid artifacts.
As we cannot put the liquid nitrogen back in the tank, it is not reused
either, though if we have several biopsies in a day we use the same
liquid nitrogen.
Frozen biopsies are stored in a little white box (EM sciences, I
think)labeled with patient name and number, in the ultrafreezer.
Best of luck with your lab.  If your pathologist is satisfied with the
method you use now you should stick to it, as I said, I will investigate
it with our cryostats here.  
Karen



Karen M. Weidenheim, M.D.
Professor of Pathology, Clinical Neurology and Clinical Neurosurgery
Albert Einstein College of Medicine
Chief, Division of Neuropathology
Montefiore Medical Center
111 East 210th Street
Bronx, NY   10467 
(718) 920-4446
FAX (718) 653-3409
Beeper (917) 556 3696
CONFIDENTIALITY NOTICE:  This email, including any attachments, is for
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the work product doctrine.  Any unauthorized review, use, disclosure or
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please contact the sender by reply e-mail and destroy all copies of the
original message.  Thank you.

>>> manal galal  7/16/2005 8:06:46 AM >>>
Hi all, 
 
  I wanted to ask about how to use liquid nitogen in freezing muscle
biopsies. I mean what does it come in? How much do I use? Do I discard
it after I use it? What kind of container do I use? How long do I put
the biopsy in it? How do I store it? 
  By the way I freeze muscle in the quick freezing chamber of the
cryostat, and am getting very little to no artifacts. But anyone that
learns of how I freeeze biopsies says that it will give me disasterous
resuls. Do you think liquid nitrogen will be superior to my old method.
 
Thanks in advance
manal

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------------------------------

Message: 14
Date: Mon, 18 Jul 2005 18:33:16 -0400
From: "Smith, Allen" 
Subject: RE: [Histonet] liquid nitrogen
To: "manal galal" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<5D2189E74151CC42BEC02906BA8996322B90CB@exchsrv01.barrynet.barry.edu>
Content-Type: text/plain;	charset="us-ascii"

    Before trying to handle liquid nitrogen, get someone who uses it to show
you how to handle it! Liquid nitrogen is very dangerous when improperly
handled. I know of 2 deaths from the abuse of liquid nitrogen. 
    Liquid nitrogen comes in a high pressure cylinder holding anything from
200 ml to 50 liters of liquid nitrogen (which will expand to 0.025 to 6
cubic meters of nitrogen gas).  The high pressure cylinder itself is
dangerous.  If it falls and the valve breaks, escaping gas will turn the
cylinder into a missile.  The escaping gas can cause severe frostbite if you
are near it.  Even at a distance, large amounts of escaping nitrogen can
displace enough air to suffocate you.  
    Small amounts of liquid nitrogen spilled on yourself will cause severe
frostbite.  Large amounts can freeze a limb solid and make it as prone to
shattering as an ice cube.  
    If you must learn by yourself, buy one of the small bottles sold for
dermatological or lecture demonstration use.  They usually contain about 50
liters of nitrogen gas compressed down to about 400 ml at 120 Atm.  Wear
nitrile gloves over leather gloves.  Make sure your work area is well
ventilated. Clamp a Dewar flask in place and release the nitrogen quickly
into the Dewar flask.  Half of the nitrogen will escape as a very cold gas.
The other half will run into the Dewar flask as a liquid.  When the Dewar
flask is half full, shut off the nitrogen.  Drop the tissue into the liquid
nitrogen in the bottom of the Dewar flask.  After a few minutes retrieve the
tissue with long forceps and put it in your cryostat.  Let the liquid
nitrogen evaporate.
    Personally, I don't like liquid nitrogen.  The layer of nitrogen gas
that forms between the tissue and the liquid nitrogen prevents efficient
freezing. If the piece of tissue is large, the outside freezes first; then
the freezing of the inside of the tissue cracks the frozen outside.  	

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of manal galal
Sent: Saturday, July 16, 2005 8:07 AM
To: Histonet@Pathology.swmed.edu
Subject: [Histonet] liquid nitrogen

Hi all, 
 
  I wanted to ask about how to use liquid nitogen in freezing muscle
biopsies. I mean what does it come in? How much do I use? Do I discard it
after I use it? What kind of container do I use? How long do I put the
biopsy in it? How do I store it? 
  By the way I freeze muscle in the quick freezing chamber of the cryostat,
and am getting very little to no artifacts. But anyone that learns of how I
freeeze biopsies says that it will give me disasterous resuls. Do you think
liquid nitrogen will be superior to my old method.
 
Thanks in advance
manal

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Barry University - Miami Shores, FL (http://www.barry.edu) 



------------------------------

Message: 15
Date: Mon, 18 Jul 2005 19:09:37 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] CAP inspections
To: "Histology SLU" ,
	
Message-ID: <000501c58bf6$26c29830$b4bd0b43@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Susan,
I had my inspection in January. Can't speak for others, but, we received 
Accreditation with Accommodation on the last two inspections. This year we 
got slammed pretty hard. I think it's because of the incident last year 
concerning a lab in Maryland. CAP gave the lab an outstanding inspection, 
but there were many QA/QC issues that the some lab employees complained to 
CLIA.
    From what I was told, CAP had to explain itself to Congress.
    My QA program was torn apart (received compliments on the same programs 
during the last two inspections). Was written up because some of the 
temperature charts had missing dates (would you rather me pencil whip 
these?).Really took it the you know what because a couple of water cultures 
were not documented.
    As a matter of fact, we're giving a lecture on "Preparing for a CAP 
Inspection" at he NSH. It's number 99, the last lecture.
    Good luck. If I can help, just drop me a line.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
From: "Histology SLU" 
To: 
Sent: Monday, July 18, 2005 12:38 PM
Subject: [Histonet] CAP inspections


> Hello All:
>
> This is directed at those of you that may have had your CAP inspection in 
> the last year.  I have been hearing that the inspectors have been, shall 
> we say, interpreting some of the checklist questions in an unusual manner. 
> My question to you all is, were there any questions where you got "tagged" 
> for something that you had always done and had passed previously?  Also, 
> can you share ANY experiences that you had through your CAP inspection 
> good or bad?  As always, thank you for your insights and opinions.  This 
> is a super group and I always appreciate your comments.
>
> Susan
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam?  Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> -- 
> No virus found in this incoming message.
> Checked by AVG Anti-Virus.
> Version: 7.0.323 / Virus Database: 267.9.1/51 - Release Date: 7/18/2005
>
> 




------------------------------

Message: 16
Date: Mon, 18 Jul 2005 19:14:31 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] How is everyone carrying out the new CAP req.?
	Is	theredocumented evidence of daily review of the
To: "Billie Zimmerman" ,
	
Message-ID: <008801c58bf7$1d4807d0$b4bd0b43@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Billie,
we send a QC sheet and an H&E control with the first set of slides. The 
sheet has a large "comments" section. The pathologists write anything in 
that space from "too many wrinkles" to  "not enough hematoxylin"
    This was accepted by the nice inspector who inspected my lab this 
January. One of the few items he did like.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX


----- Original Message ----- 
From: "Billie Zimmerman" 
To: 
Sent: Monday, July 18, 2005 12:09 PM
Subject: [Histonet] How is everyone carrying out the new CAP req.? Is 
theredocumented evidence of daily review of the


> How is everyone carrying out the new CAP req.?   Is there documented
> evidence of daily review of the technical quality of histologic
> preparations by the pathologist?
>
> This req. came out in April so I was wondering the best way to handle
> this.
>
> Thanks,
> Billie Zimmerman
> Medical College of GA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>
>
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>
> 




------------------------------

Message: 17
Date: Tue, 19 Jul 2005 07:49:00 +0100
From: "Rogerson Kemlo (ELHT) Pathology" 
Subject: RE: [Histonet] Bodies in morgue - who does the checking?
To: "Paula Wilder" ,
	
Cc: "Richardson Lorraine \(REU\) BurnleyHC"
	
Message-ID:
	<53A22BBB01D6184EBF7A4DC18A021E9E5AD49F@elht-exch1.xelht.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

In the UK I believe that it is the Police's responsibility to check in
the body, strip and take valuables (with a Porter in attendance); these
are obviously BID's (Brought in Dead). If 'more expert help' is needed,
for example a badly decomposed body or a mutilated one, then an APT
(Anatomical Pathology Technician) would be called. Hospital cases are
dealt with by the Nursing Staff and placed in the Mortuary by the
Porters.

Is this a Universal UK procedure?

-----Original Message-----
From: Paula Wilder [mailto:histo20@hotmail.com] 
Sent: 18 July 2005 17:38
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bodies in morgue - who does the checking?

Hi everyone!

Any feedback on whose responsibility it is to check bodies in the morgue
at 
your respective instituition would be greatly appreciated.  So far, by 
phoning neighboring hospitals, I have found that Security, a diener
service, 
or the Pathology Assistants are the ones responsible.  Any help in this 
would truly be greatly appreciated!  Thanks so much!

Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741



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------------------------------

Message: 18
Date: Tue, 19 Jul 2005 09:29:42 -0500
From: "Dunn-Jena, Patsy A" 
Subject: [Histonet] unsubscribe
To: "HistoNet Server" 
Message-ID:
	
	
Content-Type: text/plain;	charset="us-ascii"

 



------------------------------

Message: 19
Date: Tue, 19 Jul 2005 15:40:51 +0100
From: Jean Gillson 
Subject: [Histonet] Cytology Immuno's
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: 
Content-Type: text/plain

Can I ask you all, what do you use as controls when doing ICC on cytology
cytospin preps or smears?  Since there isn't much sample, do you use
paraffin sections as positive controls and is that adequate control
measures?  We currently only use paraffin cytoblocks but considering ICC on
thin layer cytology preps and wondering what control material to use.
Thanks for your help.



------------------------------

Message: 20
Date: Tue, 19 Jul 2005 10:56:39 -0400
From: "Habitzruther, Michael" 
Subject: [Histonet] Problem with Eosin Y staining
To: 
Cc: "Demant, Peter" 
Message-ID:
	<97101976F8A044468CA74FE11883B90E25EAAF@VISTA.roswellpark.org>
Content-Type: text/plain;	charset="iso-8859-1"

Our laboratory is having a problem with the staining of mouse tumor samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going fine until recently.  The specific problem we are having is after the cover-slip is put on and the acrymount dries, the Eosin Y stain seems to be bleeding out, leaving only the hemotoxylin staining.  A histologist from the institute suggested it may have something to do with the relative humidity in our lab, and therefore allowing the slides to set under a flow-hood may help.  Any other suggestions or protocol modifications would be greatly appreciated.  Thank you very much.


This email message may contain legally privileged and/or confidential information.  If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited.  If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.



------------------------------

Message: 21
Date: Tue, 19 Jul 2005 16:05:25 +0100
From: "Rogerson Kemlo (ELHT) Pathology" 
Subject: RE: [Histonet] Problem with Eosin Y staining
To: "Habitzruther, Michael" ,
	
Message-ID:
	<53A22BBB01D6184EBF7A4DC18A021E9E698DCB@elht-exch1.xelht.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

Eosin is soluble in both water and alcohol isn't it? Well if it bleeds
out one of these must be present mustn't it? Solution (forgive the pun)
is to eradicate both; I suspect it's alcohol.

-----Original Message-----
From: Habitzruther, Michael
[mailto:Michael.Habitzruther@RoswellPark.org] 
Sent: 19 July 2005 15:57
To: histonet@lists.utsouthwestern.edu
Cc: Demant, Peter
Subject: [Histonet] Problem with Eosin Y staining

Our laboratory is having a problem with the staining of mouse tumor
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going
fine until recently.  The specific problem we are having is after the
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to
be bleeding out, leaving only the hemotoxylin staining.  A histologist
from the institute suggested it may have something to do with the
relative humidity in our lab, and therefore allowing the slides to set
under a flow-hood may help.  Any other suggestions or protocol
modifications would be greatly appreciated.  Thank you very much.


This email message may contain legally privileged and/or confidential
information.  If you are not the intended recipient(s), or the employee
or agent responsible for the delivery of this message to the intended
recipient(s), you are hereby notified that any disclosure, copying,
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received this message in error, please notify the sender immediately by
e-mail and delete this email message from your computer. Thank you.

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------------------------------

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End of Histonet Digest, Vol 20, Issue 22
****************************************

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