Re: [Histonet] re macrophage marker


Heating methods seem to diminish or abolish the staining-  I've tried HIER as well as other enzymes and Proteinase K works the best.  
I use it at about 1 ug/ml using the following method-
 30 min in primary
30 min Rabbit anti-rat (adsorbed against mouse)
30 min labeled polymer (Envision+ HRP or Mach-2 ALP)
DAB or Permanent Fast Red
This method works great for me.

Carl Hobbs  wrote:
Bill....thanks for insight. On the look-out for just a mac marker for a PhD student's project on FFPWs of mouse lungs. Have you tried Ag retrieval other than by proteolytic digestion? ( eg HIER)
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