Re: [Histonet] Paraffin in cassette holding chamber?

From:"Jackie M O'Connor"

I've done this both ways, with and without.  I once thought that the 
cassettes had to be held in molten paraffin to ensure a homogenous block - 

I've since changed my mind. It doesn't matter.  I prefer a hot dry holding 
chamber - less messy. 

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist 
Discovery Cancer R4N2 AP3
Sent by:
07/14/2005 11:47 AM

        Subject:        [Histonet] Paraffin in cassette holding chamber?

I want to get an idea of who is (and who is not, and why) adding paraffin
to the cassette holding chamber of the embedding center.  Once
processed-How long are tissues ok to sit without paraffin on them?

All thoughts, opinions, experience is appreciated...Thanks.

Michele Marggi, HT
Surgical Pathology Supervisor
St. Marys Hospital Medical Center
707 S Mills Street
Madison WI  53715
Telephone: 608.258.6930
Fax: 608.258.6268

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