Re: [Histonet] HIER - best retrieval with minimum tissue damage?

From:"Richard Cartun"

What fixative do you use?  How long do you fix?


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Kristopher Kalleberg"  07/11/05 03:01PM >>>
I almost exclusively perform IHC on skin and always use the pressure cooker for
HIER.  I rarely have any problems or encounter disruptions in the morphology of
the sample.  I put the slides in the pressure cooker and when the pressure
reaches the appropriate level I let them cook for 6 minutes.  It seems to be a
fail-safe.  Hope this helps.

Kris Kalleberg
Unilever R&D
40 Merritt Blvd.
Trumbull CT 06611
203 381 5765

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