Re: [Histonet] Fast myosin IHC
We successfully use:
- mo-a-Myosin II (fast), clone MY-32, Sigma M 4276, working conc. approx 10
ug Ig/ml (corresponds to 1:400 with the lot we have), HIER in citrate pH 6.0
- mo-a-Myosin I (slow), clone NOQ7.5.4D, Sigma M 8421, working conc. approx
7.5 ug Ig/ml (corresponds to 1:1000 with the lot we have), HIER in citrate
pH 6.0 (pressure cooker)
Both mAbs work very well (see
http://www.pathology.unibe.ch/Diagn/speztech/spez_ihc.htm for a pic (german
Can you give me a hint in return, where you get your antibodies against
Hepatitis B surface Ag (HBs) and core Ag (HBc) from? Our source is no longer
able to supply them, due to some bureaucratic / eurocratic rules, now I'm
looking for a reliable replacement.
Many thanks in advance!
Institute of Pathology, University of Bern, Switzerland
----- Original Message -----
From: "Richard Cartun"
Sent: Tuesday, July 12, 2005 3:26 PM
Subject: [Histonet] Fast myosin IHC
> Is anyone doing immunohistochemistry for fast myosin on formalin-fixed
> human tissue? If so, whose antibody are you using? My "Pub Med" search
> did not turn up much. Thanks!
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
> Confidentiality Notice
> This e-mail message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and destroy
> all copies of the original message.
> Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>