Re: [Histonet] ADG & Dysferlin troubles

From:Dana Settembre

I use  Dysferlin from Novocastra on frozen sections, but not FITC, just
regular IHC. If you're desperate we can talk.
I use it with a detection kit for mouse, any vendor will usually do and
DAB as the chromogen. No special scope is needed.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> "Lewis, Sarah"  7/12/2005 1:48:17 PM >>>
Hello histoneters,
    I could really use any procedural information anyone has on
Alpha-dystroglycan (antibody only available thru Upstate) and Dysferlin
(antibody from VectorLabs).
        I have been attempting the stains for 2months now with no
satisfying results. I have tried several different
procedures/fixatives/incubation times/dilutions/. I perform a battery of
14 neuromuscular Immunoflourescence, they are all BEAUTIFUL fitc green
with no background staining (picture perfect) .....except these two
boogers. PLEASE HELP me make my pathologist proud!!! ALL THE CREDIT TO

Sarah E. Lewis 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 

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