RE: [Histonet] goat anti-galectin 1

From:"Elizabeth Chlipala"

Patsy

I use labeled polymers with goat primaries as follows:

After the goat antibody I use a rabbit anti-goat secondary.  I get this
from vector labs and then I use the rabbit envision + next.  It works
great.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Wednesday, July 06, 2005 8:55 AM
To: 'Histonet'
Subject: [Histonet] goat anti-galectin 1

Anybody using SC goat anti-galectin 1 antibody on ffpe tissue? If so
please
share your protocol.

Thank you,

Patsy

 

P.S.

Has anyone developed a labelled polymer type detection system to use
with
goat primary antibodies yet?

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