RE: [Histonet] Vibratome sections of unfixed tissues

From:"Charles Scouten"

Embedding would take time, at a premium with unfixed tissue.  

When used to collect brain slices for brain slice chamber electrophsyiology, researchers either just put the brain block down on wet filter paper, and get enough adhesion from that to cut, or use an instant setting cyanoacrylate provided with the Vibratome.

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 

-----Original Message-----
From: [] On Behalf Of Elizabeth Chlipala
Sent: Friday, July 15, 2005 2:59 PM
To: 'Johnson, Teri'; 'Histonet'
Subject: RE: [Histonet] Vibratome sections of unfixed tissues


We have not done unfixed tissue via vibratome, but we have done paraformaldehyde fixed and we use gelatin to create a block with the tissue in it.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Johnson, Teri
Sent: Friday, July 15, 2005 12:36 PM
To: Histonet
Subject: [Histonet] Vibratome sections of unfixed tissues

How does one mount unfixed tissues for vibratome sectioning?  Are they encased in any medium such as agarose or gelatin, or simply glued onto the stage and sectioned?  I can't seem to find information on this anywhere.

Thanks, and have a great weekend all!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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