RE: [Histonet] Vibratome sections of unfixed tissues

From:"Elizabeth Chlipala"


We have not done unfixed tissue via vibratome, but we have done
paraformaldehyde fixed and we use gelatin to create a block with the
tissue in it.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Johnson,
Sent: Friday, July 15, 2005 12:36 PM
To: Histonet
Subject: [Histonet] Vibratome sections of unfixed tissues

How does one mount unfixed tissues for vibratome sectioning?  Are they
encased in any medium such as agarose or gelatin, or simply glued onto
the stage and sectioned?  I can't seem to find information on this

Thanks, and have a great weekend all!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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