My co-worker just successfully ran a predilute polyclonal ALK from
BioCare Medical on our Benchmark with an MCC1, 32"ttn and no  block.  We
had previously only run it on the Nexes and haven't run it on our XT
yet.  
Unfortunately, we are not flush w/ control tissue (we only have 1
positive case from 1999) so I can't send you a block.  However, would a
few cut slides help you get started?
I know that when I first worked up this antibody I didn't have much luck
w/ the rabbit monoclonals I tried.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Malam
Jacqueline
Sent: Friday, July 15, 2005 3:21 AM
To: Histonet submissions (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Ventana Benchmark XT and ALK 1


Does anyone out there do ALK 1 on the Ventana Benchmark? If so, I would
be grateful if you could let me know what clone you use, what the
protocol and dilution are, and if you could spare me a positive control
block. 
I have tried 2 clones - Dako's ALK 1 and Lab Vision's SP8 but to no
avail; I have had experience of certain clones of some antisera
(Vector's CD 138 and Dako's calretinin for example) not working on the
Benchmark and it's possible that these clones are not suitable. Having
said that, we only had 2 cases and possibly they may have been negative
for ALK 1 anyway. I used the lowest recommended dilution, both the mild
and standard heat protocols, and the 3 primary incubation temperatures
(room, 37 and 42) and even tried protease and no retrieval but got
nowhere. I have also tried to get a known positive control block but no
one I've tried has one! All assistance appreciated! Jacqui Malam Royal
Infirmary Lancaster England



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