RE: [Histonet] Proteinase K

From:"Elizabeth Chlipala"

Guofeng

I have used Dako's proteinase K for quite a few antibodies on FFPE
decalcifed bone sections.  The diluent of the Dako reagent is listed on
the spec sheet its 0.05mol/L Tris-HCL, 0.015mol/L sodium azide, pH 7.5.

Depending upon the antibody, I use the proteinase K for 2 to 5 minutes.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pex
Sent: Monday, July 11, 2005 6:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Proteinase K

Hello, all,
 
I plan to use proteinase k from Dakocytomation for antigen retrieval in
bone paraffin sections, but the specification only shows proteolytic
enzyme solution diluted in 0.05 mol/L Tris-Hcl, not the concentrations
in details, so I have no ideas, if you have some experience about it, I
hope you can do me a favor!Thank you!
 
 
Guofeng

		
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