I do not put any extra paraffin into the holding chamber. If I am not
going to embed right away I take the cassettes out of the processor and
let them cool off with the paraffin hardening around it. I have not had
any complaints with tissue handled in this way.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Avenue
Houston, TX 77030
832-355-6524
832-355-6812 ( fax)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Michele_Marggi@ssmhc.com
Sent: Thursday, July 14, 2005 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin in cassette holding chamber?

I want to get an idea of who is (and who is not, and why) adding
paraffin
to the cassette holding chamber of the embedding center.  Once
processed-How long are tissues ok to sit without paraffin on them?

All thoughts, opinions, experience is appreciated...Thanks.

Michele Marggi, HT
Surgical Pathology Supervisor
St. Marys Hospital Medical Center
707 S Mills Street
Madison WI  53715
Telephone: 608.258.6930
Fax: 608.258.6268


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