If it's going to be a really long time (or even to hold extra tissue,
unembedded for a great length of time) just pull the cassettes out and then
put them back in to heat up right before embedding.

Bonnie Whitaker


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel
V
Sent: Thursday, July 14, 2005 2:33 PM
To: Michele_Marggi@ssmhc.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin in cassette holding chamber?


We do not add paraffin to our holding chamber.   But they do not sit
there very long, as we start embedding right away.   Is there a reason
you want them to sit for a time before embedding? 


Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital - Changing Children's Lives

Phone - 501.364.4240
Fax - 501.364.3912 

Visit us on the web at      www. archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Michele_Marggi@ssmhc.com
Sent: Thursday, July 14, 2005 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin in cassette holding chamber?

I want to get an idea of who is (and who is not, and why) adding paraffin to
the cassette holding chamber of the embedding center.  Once processed-How
long are tissues ok to sit without paraffin on them?

All thoughts, opinions, experience is appreciated...Thanks.

Michele Marggi, HT
Surgical Pathology Supervisor
St. Marys Hospital Medical Center
707 S Mills Street
Madison WI  53715
Telephone: 608.258.6930
Fax: 608.258.6268


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