That's great, never had the time to work it out myself....
approximate working concentration/dilution and incubation?
Much appreciated
regards Luis

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bill
Sent: Tuesday, July 12, 2005 9:29 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Macrophage marker mouse tissue


In my experience, Serotec's CD68 clone FA-11 is a much better tissue
macrophage marker than F4/80, which is not expressed on all macs (i.e.
lung).   A 5 min. pretreatment w/ Dako Prot K is all that is required on
FFPE mouse tissue.


"Edwards, R.E."  wrote:F4/80

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of GT Hebert
Sent: 11 July 2005 17:30
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Macrophage marker mouse tissue


Hello,

I am in need of a good macrophage marker on FFPE tissue. I will be using it
on mouse tissue... mainly the heart (but other tissues may be included). So
far I have looked into a number of antibodies (CD68, Mac-2) but can never be
certain which one to choose.

Thank you in advance for your help.

Sincerely,

Gustave



Gustave Hebert, HT (ASCP)
Scientist II
Wyeth Research
Cardiovascular and Metabolic Diseases
Cambridge MA


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