RE: [Histonet] Macrophage marker mouse tissue

From:Luis Chiriboga

A couple of years ago, I used (in frozen mouse heart) Serotec (cat#
MCA1957s) rat anti-mouse cd68 which targets macrosialin, the murine CD68
homologue. It's a monoclonal (FA-11) IgG2a, detected  with a biotinylated
rabbit anti-rat (mouse adsorbed) followed by SA-alkaline phosphatase red.
It worked very nicely but at a very high concentration. At the time they had
not determined whether it was viable in FFPE so you may want to give them a
call and see.
Hope this helps
Regards Luis

-----Original Message-----
[]On Behalf Of GT Hebert
Sent: Monday, July 11, 2005 12:30 PM
Subject: [Histonet] Macrophage marker mouse tissue


I am in need of a good macrophage marker on FFPE tissue.  I will be using it
on mouse tissue... mainly the heart (but other tissues may be included).  So
far I have looked into a number of antibodies (CD68, Mac-2) but can never be
certain which one to choose.

Thank you in advance for your help.



Gustave Hebert, HT (ASCP)
Scientist II
Wyeth Research
Cardiovascular and Metabolic Diseases
Cambridge MA

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