RE: [Histonet] Macrophage marker mouse tissue

From:"Elizabeth Chlipala"


We use F4/80 as a macrophage marker in mice.  We have also tried MAC-2.
Both work fine on FFPE tissue.  Most of our clients ask for the F4/80
marker.  We purchase ours from serotec.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of GT
Sent: Monday, July 11, 2005 9:30 AM
Subject: [Histonet] Macrophage marker mouse tissue

I am in need of a good macrophage marker on FFPE tissue.  I will be
using it on mouse tissue... mainly the heart (but other tissues may be
included).  So far I have looked into a number of antibodies (CD68,
Mac-2) but can never be certain which one to choose.
Thank you in advance for your help.
Gustave Hebert, HT (ASCP)
Scientist II
Wyeth Research
Cardiovascular and Metabolic Diseases
Cambridge MA

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