RE: [Histonet] H&E after IHC
Hematoxylin is an acceptable counterstain for immunoperoxidase techniques
(though I personally prefer methyl green). I would avoid eosin however.
The brown color that develops in such techniques has a definite reddish
cast, and using a background stain in the red range could interfere with
interpretation of results, especially in weakly stained areas.
> From: firstname.lastname@example.org on behalf of wen
> Sent: Wednesday, July 13, 2005 11:13 AM
> To: histonet
> Subject: [Histonet] H&E after IHC
> Hi histonetters,
> I have to ask a basic question. I am going to do IHC using DAB. I used to
> do hematoxylin counterstaining. Can I do H&E after IHC? Will it cover
> brown color of DAB?
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