[Histonet] no nuclei in frozen sections
Hello again everyone!
I need your advice and suggestions again! I have begun to have holes in
frozen sections where the nuclues should be. I am cutting clinically
engineered skin specimens, at -21. NO flash freeze spray. The specimens
are cut at 5-6 microns and are embedded in Shandons' M-1 embedding matrix.
This problem seems to have come up in the last 3 weeks or so, but I cannot
unravel why. Nothing has been changed on my end, but the problem has been
in all the researchers submissions. I have done recuts and they are still
there. I have wracked my brain and am coming up clueless! I have contacted
the manufacturer for additional information on the properties of M-1, but I
am still coming up with no answers. Thanks in advance,
Shriners Hospital for Children
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