[Histonet] mouse brain
Hello, please have any of you who process & section paraformaldehyde
fixed, paraffin embedded mouse brain experienced asymmetry of the right &
left hemispheres? Recently our coronal mouse brain sections which appear
symmetrical upon gross trim and after initial facing of paraffin are
asymmetrical after staining. I've rotated the block holder to the limit and
sectioned the most rostral hemisphere at higher micron thickness to
accommodate. However, to my dismay and disappointment none of this has
helped very much. Any pointers in remedying this situation ASAP will be
greatly appreciated. Thanks, Atoska
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