[Histonet] liver cryosections
|From:||"Stephen Peters M.D." |
I agree with the others who have suggested that the tissue is too cold. My frozen section tutorial has some pics of shattered sections on this page so you can see compare your results to these over-cooled sections.
Liver is one of the easier tissues to cut. It will cut best at the warmest temp that it will cut at all. Get your blocks down to cryostat temp and then use your thumb to warm it to a point where it cuts without shattering.
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