[Histonet] bone-marrow decal

From:"Gudrun Lang"

Hi,

What duration of fixation is recommanded for bonemarrow trephines (3 mm
diameter), if you use a formicacid-formaldehyd solution subsequently for
decalcification?

 

Please, can anyone share his/her experience in this field. (morphology, ihc)

 

Our protocol:  ca. 20 hours 9% buffered formaldehyd; then 6-8 hours decal
with the combinated solution.

 

Thank you

Gudrun



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