[Histonet] University of Pittsburgh
From: | "Stephen Peters M.D." |
Are the nuclei totally missing? or are there holes in the nuclei? If there are holes in the nuclei
I believe this is one of the manifestations of freeze artefact which becomes very obvious
in sections in the under 3 micra range. Even though a cryostat is set for 5 micra it is very
common for the first few sections to be different thickness, commonly a thick one followed
by a thin one.
I show pics of what I believe are nuclear ice crystals and the effect of section thickness on
this page of my frozen section tutorial: http://pathologyinnovations.com/new_page_3.htm.
If the nuclei are falling out, I have no answer.
Stephen
Stephen Peters M.D.
Vice Chairman of Pathology
Hackensack University Medical Center
201 996 4836
Pathology Innovations, LLC
410 Old Mill Lane,
Wyckoff, NJ 07481
201 847 7600
www.pathologyinnovations.com
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