[Histonet] University of Pittsburgh

From:"Stephen Peters M.D."

Are the nuclei totally missing? or are there holes in the nuclei? If there are holes in the nuclei 
I believe this is one of the manifestations of freeze artefact which becomes very obvious 
in sections in the under 3 micra range. Even though a cryostat is set for 5 micra it is very
 common for the first few sections to be different thickness, commonly a thick one followed 
by a thin one. 
I show pics of what I believe are nuclear ice crystals and the effect of section thickness on
 this page of my frozen section tutorial:  http://pathologyinnovations.com/new_page_3.htm.
If the nuclei are falling out, I have no answer.

Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 

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